ArticleThe Journal of clinical investigation1994
Human clusterin gene expression is confined to surviving cells during in vitro programmed cell death.
Article in The Journal of clinical investigation, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 166 citations in OpenAlex.
- Deiminated proteins in extracellular vesicles and serum of llama (Lama glama)-Novel insights into camelid immunity.Molecular immunology · 2020Article
- Clusterin increases mitochondrial respiratory chain complex I activity and protects against hexavalent chromium-induced cytotoxicity in L-02 hepatocytes.Toxicology research · 2019Article
- Ascorbate induces apoptosis in melanoma cells by suppressing Clusterin expression.Scientific reports · 2017Article
- Characterization of Vitreous and Aqueous Proteome in Humans With Proliferative Diabetic Retinopathy and Its Clinical Correlation.Proteomics insights · 2017Article
- Hepatitis D and hepatocellular carcinoma.World journal of hepatology · 2015Review
- Alzheimer risk variant CLU and brain function during aging.Biological psychiatry · 2013Article
- Article
- Plasma clusterin concentration is associated with longitudinal brain atrophy in mild cognitive impairment.NeuroImage · 2012Article
- Identification of clusterin domain involved in NF-kappaB pathway regulation.The Journal of biological chemistry · 2010Article
- Genome-wide transcription analysis of histidine-related cataract in Atlantic salmon (Salmo salar L).Molecular vision · 2009Article
- Clusterin in human gut-associated lymphoid tissue, tonsils, and adenoids: localization to M cells and follicular dendritic cells.Histochemistry and cell biology · 2008Article
- Clusterin expression in non-neoplastic adenohypophyses and pituitary adenomas: cytoplasmic clusterin localization in adenohypophysis is related to aging.Endocrine pathology · 2008Article
- Article
- Two thyroid hormone-mediated gene expression patterns in vivo identified by cDNA expression arrays in rat.Nucleic acids research · 2001Article
- Levamisole induced apoptosis in cultured vascular endothelial cells.British journal of pharmacology · 2000Article
- Overexpression of clusterin in human breast carcinoma.The American journal of pathology · 2000Article
- Estrous cycle regulation of mammary epithelial cell proliferation, differentiation, and death in the Sprague-Dawley rat: a model for investigating the role of estrous cycling in mammary carcinogenesis.Journal of mammary gland biology and neoplasia · 2000Article
- Nerve growth factor and epidermal growth factor stimulate clusterin gene expression in PC12 cells.The Biochemical journal · 1999Article
- Stress-induced transcription of the clusterin/apoJ gene.The Biochemical journal · 1997Article
- Mildly oxidized LDL induces an increased apolipoprotein J/paraoxonase ratio.The Journal of clinical investigation · 1997Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Clusterin is a serum glycoprotein endowed with cell aggregating, complement inhibitory, and lipid binding properties, and is also considered as a specific marker of dying cells, its expression being increased in various tissues undergoing programmed cell death (PCD). However, no study has so far directly shown that cells expressing clusterin in these tissues are actually apoptotic as defined by morphological and biochemical criteria. We have studied cellular clusterin gene expression in vitro using three different models of PCD: (a) ultraviolet B (UV-B) irradiation of human U937, HeLa, and A431 cell lines, (b) in vitro aging of human peripheral blood neutrophils (PMNs), and (c) dexamethasone-induced cell death of the human lymphoblastoid cell line CEM-C7. In all three models, the classical morphological and biochemical features of PCD observed did not correlate with an increase, but with either a marked decrease or an absence of clusterin gene expression as assessed by Northern blot analysis. In situ hybridization of U937 and A431 cells after UV-B irradiation revealed, in addition, that only morphologically normal cells that are surviving continue to express the clusterin gene. Our results demonstrate that in the human myeloid, lymphoid, and epithelial cell types studied, clusterin gene expression is not a prerequisite to their death by apoptosis. In addition, and most interestingly, in situ hybridization of U937 and A431 cells revealed that only surviving cells express the clusterin gene after the induction of PCD, thus providing novel evidence suggesting that clusterin may be associated with cell survival within tissues regressing as a consequence of PCD.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.