ArticleThe Journal of clinical investigation1994
Cellular mechanism of the conduction abnormalities induced by serum from anti-Ro/SSA-positive patients in rabbit hearts.
Article in The Journal of clinical investigation, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 147 citations in OpenAlex.
- Autoimmune antibodies in arrhythmia: a narrative review of potential therapeutic targets to prevent overtreatment.Cardiovascular diagnosis and therapy · 2025Review
- Myositis-associated antibodies predict the severity of lung involvement in adult patients with inflammatory myositis - a cohort study of 70 adult patients with myositis in a single center.Frontiers in medicine · 2024Article
- Affinity purification of serum-derived anti-IA-2 autoantibodies in type 1 diabetes using a novel MBP-IA-2 fusion protein.Biochemistry and biophysics reports · 2023Article
- Clinical Characteristics and Mid-term Follow-up in Children with Isolated Complete Atrioventricular Block.Anatolian journal of cardiology · 2023Article
- Complete heart block in neonatal lupus: a forgotten cause of fetal bradycardia.BMJ case reports · 2021Article
- Autoimmune Calcium Channelopathies and Cardiac Electrical Abnormalities.Frontiers in cardiovascular medicine · 2019Review
- Association of Anti-Ro/Sjögren's syndrome type A Antibodies and Complete Atrioventricular Block in an Adult With Sjögren's Syndrome.Archives of rheumatology · 2018Article
- Article
- Congenital heart block: evidence for a pathogenic role of maternal autoantibodies.Arthritis research & therapy · 2012Review
- RGS Proteins in Heart: Brakes on the Vagus.Frontiers in physiology · 2012Article
- Clinical and pathological roles of Ro/SSA autoantibody system.Clinical & developmental immunology · 2012Review
- Complete atrioventricular block in adult Sjögren's syndrome with anti-Ro autoantibody.The Korean journal of internal medicine · 2011Review
- Pregnancy outcomes in patients with autoimmune diseases and anti-Ro/SSA antibodies.Clinical reviews in allergy & immunology · 2011Review
- Role of calcium channels in congenital heart block.Scandinavian journal of immunology · 2010Review
- Congenital heart block: identification of autoantibody binding site on the extracellular loop (domain I, S5-S6) of alpha(1D) L-type Ca channel.Journal of autoimmunity · 2010Article
- Cardiac manifestations of neonatal lupus erythematosus: guidelines to management, integrating clues from the bench and bedside.Nature clinical practice. Rheumatology · 2009Review
- Identification and management of fetuses at risk for, or affected by, congenital heart block associated with autoantibodies to SSA (Ro), SSB (La), or an HsEg5-like autoantigen.Rheumatology international · 2006Article
- 52-kDa Ro/SSA epitopes preferentially recognized by antibodies from mothers of children with neonatal lupus and congenital heart block.Arthritis research & therapy · 2006Article
- Neonatal lupus: clinical features and management.Paediatric drugs · 2004Review
- Neonatal lupus.Clinical reviews in allergy & immunology · 2002Review
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
In this study, IgG fractions from sera of SLE patients with anti-Ro/SSA or anti-Ro/SSA and anti-La/SSB activity were tested in Langendorff preparations of adult rabbit hearts, aiming to reproduce the cardiac manifestations observed in neonatal lupus in an experimental model. The hearts were perfused with normal Tyrode's solution for 30 min, followed by perfusion with Tyrode's containing 0.3 mg/ml of anti-Ro/SSA- (or anti-Ro/La-) positive IgG (nine sera), anti-ribonucleoprotein (RNP)-positive IgG (five sera), or IgG fractions from normal donors (five sera). In one third of the experiments done with anti-Ro/La-positive IgG, heart block was observed. With the remaining fractions, a decrease in heart rate of 17.1% was observed, but normal sinus rhythm was maintained. The IgG fractions with anti-RNP activity (five experiments) and from normal sera (six experiments) reduced heart rates by 12.9 and 3.3%, respectively, but heart block was not observed. To further characterize the cellular mechanisms involved in the conduction disturbances observed in the whole rabbit hearts, we conducted experiments with ventricular myocytes isolated from young rabbit hearts, studied by whole cell patch-clamp technique. In these experiments, the slow inward currents were analyzed during the superfusion of the cell with normal Tyrode's solution and 5 min after superfusion with Tyrode's solution containing 0.3 mg/ml of anti-Ro/SSA- (or anti-Ro/La-) positive IgG (five sera), anti-RNP-positive IgG (three sera), or IgG from normal donors (four sera). Resting and action potential amplitudes were not affected by any of the sera used. The anti-Ro/SSA IgG fraction induced a mean reduction in the peak slow inward current of 31.6%. IgG fractions with anti-RNP activity reduced slow inward current by 4.4%, whereas IgG fractions from normal donors increased this current by 3.3%. IgG-free fractions from sera of patients with anti-Ro/SSA activity did not alter the peak slow inward current. These results show, for the first time, that the presence of anti-Ro/SSA or anti-Ro/SSA and anti-La/SSB antibody activity in IgG fractions from lupus patients' sera can induce cardiac conduction disorders similar to those observed in neonatal lupus.
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