ArticleThe Journal of clinical investigation1994
High efficiency of muscle regeneration after human myoblast clone transplantation in SCID mice.
Article in The Journal of clinical investigation, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 159 citations in OpenAlex.
- Generation of human myogenic progenitors from pluripotent stem cells for in vivo regeneration.Cellular and molecular life sciences : CMLS · 2022Review
- Current Strategies for the Regeneration of Skeletal Muscle Tissue.International journal of molecular sciences · 2021Review
- Skeletal muscle cell transplantation: models and methods.Journal of muscle research and cell motility · 2020Article
- Holistic Approach of Swiss Fetal Progenitor Cell Banking: Optimizing Safe and Sustainable Substrates for Regenerative Medicine and Biotechnology.Frontiers in bioengineering and biotechnology · 2020Article
- Vascular Delivery of Allogeneic MuStem Cells in Dystrophic Dogs Requires Only Short-Term Immunosuppression to Avoid Host Immunity and Generate Clinical/Tissue Benefits.Cell transplantation · 2018Article
- Skeletal Muscle Regenerative Potential of Human MuStem Cells following Transplantation into Injured Mice Muscle.Molecular therapy : the journal of the American Society of Gene Therapy · 2018Article
- Obestatin Increases the Regenerative Capacity of Human Myoblasts Transplanted Intramuscularly in an Immunodeficient Mouse Model.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- The emerging role of viral vectors as vehicles for DMD gene editing.Genome medicine · 2016Review
- Donor satellite cell engraftment is significantly augmented when the host niche is preserved and endogenous satellite cells are incapacitated.Stem cells (Dayton, Ohio) · 2012Article
- Intramuscular Transplantation of Muscle Precursor Cells over-expressing MMP-9 improves Transplantation Success.PLoS currents · 2011Article
- Are human and mouse satellite cells really the same?The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2010Review
- Intramuscular transplantation of human postnatal myoblasts generates functional donor-derived satellite cells.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- In vivo fluorescence imaging of muscle cell regeneration by transplanted EGFP-labeled myoblasts.Molecular therapy : the journal of the American Society of Gene Therapy · 2010Article
- Aldehyde dehydrogenase activity identifies a population of human skeletal muscle cells with high myogenic capacities.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Myogenic endothelial cells purified from human skeletal muscle improve cardiac function after transplantation into infarcted myocardium.Journal of the American College of Cardiology · 2008Article
- Human muscular fetal cells: a potential cell source for muscular therapies.Pediatric surgery international · 2008Article
- A novel myogenic cell line with phenotypic properties of muscle progenitors.Journal of molecular medicine (Berlin, Germany) · 2008Article
- Normal growth and regenerating ability of myoblasts from unaffected muscles of facioscapulohumeral muscular dystrophy patients.Gene therapy · 2005Article
- Intact satellite cells lead to remarkable protection against Smn gene defect in differentiated skeletal muscle.The Journal of cell biology · 2003Article
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Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
SCID mouse tibialis anterior muscles were first irradiated to prevent regeneration by host myoblasts and injected with notexin to damage the muscle fibers and trigger regeneration. The muscles were then injected with roughly 5 million human myoblasts. 1 mo later, 16-33% of the normal number of muscle fibers were present in the injected muscle, because of incomplete regeneration. However, > 90% of these muscle fibers contained human dystrophin. Some newly formed muscle fibers had an accumulation of human dystrophin and desmin on a part of their membrane. Such accumulations have been demonstrated at neuromuscular junctions before suggesting that the new muscle fibers are innervated and functional. The same pool of clones of human myoblasts produced only < or = 4% of muscle fibers containing human dystrophin when injected in nude mice muscles. Several of the human myoblasts did not fuse and remained in interstitial space or tightly associated with muscle fibers suggesting that some of them have formed satellite cells. Moreover, cultures of 98% pure human myoblasts were obtained from transplanted SCID muscles. In some mice where the muscle regeneration was not complete, the muscle fibers containing human dystrophin also expressed uniformly HLA class 1, confirming that the fibers are of human origin. The presence of hybrid muscle fibers containing human dystrophin and mouse MHC was also demonstrated following transplantation. These results establish that in absence of an immune reaction, transplanted human myoblasts participate to the muscle regeneration with a high degree of efficacy even if the animals were killed only 1 mo after the transplantation.
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