Evidence map›Paper›PMID 8112222›Full record

ReviewEuropean biophysics journal : EBJ1993

Total internal reflection fluorescence microscopy: application to substrate-supported planar membranes.

N L Thompson, K H Pearce, H V Hsieh

Abstract readReview
PubMed Publisher
In one paragraph

Review in European biophysics journal : EBJ, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.

0numbers the graph read from it
0cells of the map it votes in
19citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

19 citing papers in PubMed.

  1. Review
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  6. Review
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  8. Double cushions preserve transmembrane protein mobility in supported bilayer systems.Langmuir : the ACS journal of surfaces and colloids · 2008
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  17. Direct in vivo measurement of targeted binding in a human tumor xenograft.Proceedings of the National Academy of Sciences of the United States of America · 1997
    Article
  18. Article
  19. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

N L ThompsonDepartment of Chemistry, University of North Carolina, Chapel Hill 27599-3290.
K H Pearce
H V Hsieh

Funding

MOLECULAR DYNAMICS IN RECEPTOR-MEDIATED PHAGOCYTOSISR01GM037145 · NIGMS · UNIVERSITY OF NORTH CAROLINA CHAPEL HILL · PI THOMPSON, NANCY L. · 1986 to 1997
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CELL SURFACE DYNAMICS OF IGG FC RECEPTOR INTERACTIONSR55GM037145 · NIGMS · UNIVERSITY OF NORTH CAROLINA CHAPEL HILL · PI THOMPSON, NANCY L. · 1998 to 1998
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NIGMS NIH HHS GM-37145
6 · The paper itself

Abstract

The use of total internal reflection illumination in fluorescence microscopy (TIRFM) is reviewed with emphasis on application to fluorescent macromolecules that specifically and reversibly bind to planar model membranes supported on glass or quartz substrates. Several methods for characterizing macromolecular motion and organization are discussed: the measurement of equilibrium binding curves to obtain values for equilibrium binding constants; the measurement of fluorescence photobleaching recovery curves to obtain values of kinetic rate constants and surface diffusion coefficients; and the measurement of fluorescence intensities as a function of the evanescent field polarization to characterize orientational order. Applications to cell-substrate contact regions are summarized and future directions of TIRFM are outlined.

Indexed as

Membranes, ArtificialMicroscopy, FluorescenceChemical PhenomenaChemistry, PhysicalFluorescenceMembranes, Artificial

Identifiers

PMID8112222

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.