ArticlePlant molecular biology1994
A 20 nucleotide upstream element is essential for the nopaline synthase (nos) promoter activity.
Article in Plant molecular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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23 citing papers in PubMed.
- The vascular targeted citrus FLOWERING LOCUS T3 gene promotes non-inductive early flowering in transgenic Carrizo rootstocks and grafted juvenile scions.Scientific reports · 2020Article
- Effect ofFrontiers in plant science · 2017Article
- Multi-Omics Approach Identifies Molecular Mechanisms of Plant-Fungus Mycorrhizal Interaction.Frontiers in plant science · 2015Article
- Identification of a novel jasmonate-responsive element in the AtJMT promoter and its binding protein for AtJMT repression.PloS one · 2013Article
- Isolation and functional analysis of the Catharanthus roseus deacetylvindoline-4-O-acetyltransferase gene promoter.Plant cell reports · 2010Article
- Strategies for development of functionally equivalent promoters with minimum sequence homology for transgene expression in plants: cis-elements in a novel DNA context versus domain swapping.Plant physiology · 2003Article
- A xenobiotic-stress-activated transcription factor and its cognate target genes are preferentially expressed in root tip meristems.Plant molecular biology · 2000Article
- Cooperative DNA binding and sequence discrimination by the Opaque2 bZIP factor.The Plant cell · 1998Article
- An as-1-like motif controls the level of expression of the gene for the pathogenesis-related protein 1a from tobacco.Plant molecular biology · 1998Article
- The maize gene liguleless2 encodes a basic leucine zipper protein involved in the establishment of the leaf blade-sheath boundary.Genes & development · 1998Article
- DNA-binding properties, genomic organization and expression pattern of TGA6, a new member of the TGA family of bZIP transcription factors in Arabidopsis thaliana.Plant molecular biology · 1997Article
- Promoter elements controlling developmental and environmental regulation of a tobacco ribosomal protein gene L34.Plant molecular biology · 1996Article
- Coordinated activation of as-1-type elements and a tobacco glutathione S-transferase gene by auxins, salicylic acid, methyl-jasmonate and hydrogen peroxide.Plant molecular biology · 1996Article
- A multiple-stimuli-responsive as-1-related element of parA gene confers responsiveness to cadmium but not to copper.Plant physiology · 1996Article
- Auxin-sensitive elements from promoters of tobacco GST genes and a consensus as-1-like element differ only in relative strength.Plant physiology · 1996Article
- Interactions between distinct types of DNA binding proteins enhance binding to ocs element promoter sequences.The Plant cell · 1995Article
- 5'-regulatory region of Agrobacterium tumefaciens T-DNA gene 6b directs organ-specific, wound-inducible and auxin-inducible expression in transgenic tobacco.Plant molecular biology · 1995Article
- Induction of nopaline synthase promoter activity by H2O2 has no direct correlation with salicylic acid.Plant physiology · 1995Article
- Promoter analysis of the auxin-regulated tobacco glutathione S-transferase genes Nt103-1 and Nt103-35.Plant molecular biology · 1995Article
- Composite structure of auxin response elements.The Plant cell · 1995Article
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4 authors.
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Abstract
The nopaline synthase (nos) promoter is expressed in a wide range of plant cell types and regulated by various developmental and environmental factors. The nos upstream control region essential for this regulation was studied by means of synthetic oligomers using transient and stable transformation systems. Insertion of a 20 nucleotide sequence containing two hexamer motifs and a spacer region into deletion mutants lacking the upstream control region was essential for promoter activity. Mutation of one or more nucleotides of either hexamer sequence significantly altered the strength of expression of the nos promoter. Point mutations within the spacer region also strongly influenced promoter strength. Insertion of multiple copies of the 20 nucleotide sequence into the nonfunctional deletion mutants proportionally increased the promoter activity. These results suggest that this twenty nucleotide sequence is essential for the nos promoter to function. Substitution of the nos element with the ocs or 35S as-1 which contain similar hexamer motifs restored not only promoter activity but also responses to wounding, auxin, methyl jasmonate, and salicylic acid.
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