ArticleProceedings of the National Academy of Sciences of the United States of America1994
Inhibition of PC12 cell redox activity is a specific, early indicator of the mechanism of beta-amyloid-mediated cell death.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 86 papers.
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Who cites it
86 citing papers in PubMed, 412 citations in OpenAlex.
- In vitro, cellular and in vivo studies of amyloid oligomers structure and toxicity: Challenges and advances.Protein science : a publication of the Protein Society · 2026Review
- Amyloid-β and Mitochondrial Membranes: A Missing Link in Alzheimer's Pathogenesis.Molecular neurobiology · 2026Review
- Oxytocin Protects PC12 Cells Against β-Amyloid-Induced Cell Injury.Pharmaceuticals (Basel, Switzerland) · 2025Article
- Mechanisms of ARA290 in counteracting cadmium-triggered neurotoxicity in PC12 cells.Toxicology research · 2025Article
- The Role of Non-Coding RNAs in Mitochondrial Dysfunction of Alzheimer's Disease.Journal of molecular neuroscience : MN · 2024Review
- Type I Hsp40s/DnaJs aggregates exhibit features reminiscent of amyloidogenic structures.The FEBS journal · 2024Article
- Controlling Amyloid Beta Peptide Aggregation and Toxicity by Protease-Stable Ligands.ACS bio & med chem Au · 2023Article
- Tuning the surface charge of phospholipid bilayers inhibits insulin fibrilization.Colloids and surfaces. B, Biointerfaces · 2022Article
- Linking the Amyloid, Tau, and Mitochondrial Hypotheses of Alzheimer's Disease and Identifying Promising Drug Targets.Biomolecules · 2022Review
- Quantitative evaluation of the dynamic activity of HeLa cells in different viability states using dynamic full-field optical coherence microscopy.Biomedical optics express · 2021Article
- Mito-Tempo suppresses autophagic flux via the PI3K/Akt/mTOR signaling pathway in neuroblastoma SH-SY5Y cells.Heliyon · 2021Article
- Roles of Phase Separation for Cellular Redox Maintenance.Frontiers in genetics · 2021Review
- Carvacrol and Thymol Attenuate Cytotoxicity Induced by Amyloid β25-35 via Activating Protein Kinase C and Inhibiting Oxidative Stress in PC12 Cells.Iranian biomedical journal · 2020Article
- Affected albumin endocytosis as a new neurotoxicity mechanism of amyloid beta.AIMS neuroscience · 2020Article
- Structure and Neuroprotective Effect of Polysaccharide from Viscera Autolysates of SquidMarine drugs · 2019Article
- Effect of IAPP on the proteome of cultured Rin-5F cells.BMC biochemistry · 2018Article
- Global analysis of ginsenoside Rg1 protective effects in β-amyloid-treated neuronal cells.Journal of ginseng research · 2017Article
- Hormetic effect of panaxatriol saponins confers neuroprotection in PC12 cells and zebrafish through PI3K/AKT/mTOR and AMPK/SIRT1/FOXO3 pathways.Scientific reports · 2017Article
- Uptake and Toxicity of Copper Oxide Nanoparticles in C6 Glioma Cells.Neurochemical research · 2016Article
- Modulation of Amyloid β-Protein (Aβ) Assembly by Homologous C-Terminal Fragments as a Strategy for Inhibiting Aβ Toxicity.ACS chemical neuroscience · 2016Review
26 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An in vitro tissue culture cell model system for investigating the biochemical mechanisms involved in the neurodegenerative actions of beta-amyloid has been established. Using rat pheochromocytoma PC12 cells, it was found that an early, specific response of cells to the beta-amyloid protein or the beta-amyloid fragment 25-35 was a potent inhibition of cellular redox activity, as measured by 3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide (MTT) reduction. This inhibitory response was rapid and occurred at nanomolar concentrations of peptide, concentrations at which no equivalent decreases in cell proliferation or cell survival were observed. The inhibition of PC12 cell MTT reduction was initially reversible upon removal of the peptide; if sustained for several days, however, by repeated peptide application, it became associated with a dramatic reduction in cell survival. Inhibition of MTT reduction may, therefore, be an early indicator of the mechanism of beta-amyloid-mediated cell death.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.