ArticleProceedings of the National Academy of Sciences of the United States of America1994
In vivo and in vitro analysis of electrical activity-dependent expression of muscle acetylcholine receptor genes using adenovirus.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 77 citations in OpenAlex.
- hnRNP H enhances skipping of a nonfunctional exon P3A in CHRNA1 and a mutation disrupting its binding causes congenital myasthenic syndrome.Human molecular genetics · 2008Article
- Implications of activity-dependent neurotransmitter-receptor matching.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2008Review
- Activity-dependent gene regulation in skeletal muscle is mediated by a histone deacetylase (HDAC)-Dach2-myogenin signal transduction cascade.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Accelerated response of the myogenin gene to denervation in mutant mice lacking phosphorylation of myogenin at threonine 87.Molecular and cellular biology · 2004Article
- Nonneurotropic adenovirus: a vector for gene transfer to the brain and gene therapy of neurological disorders.International review of neurobiology · 2003Review
- Sodium channel mRNAs at the neuromuscular junction: distinct patterns of accumulation and effects of muscle activity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Synapse-specific and neuregulin-induced transcription require an ets site that binds GABPalpha/GABPbeta.Genes & development · 1998Article
- Implication of a multisubunit Ets-related transcription factor in synaptic expression of the nicotinic acetylcholine receptor.The EMBO journal · 1998Article
- Myasthenia gravis as a prototype autoimmune receptor disease.Immunologic research · 1997Review
- Identification and characterization of a 47 base pair activity-dependent enhancer of the rat nicotinic acetylcholine receptor delta-subunit promoter.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Therapeutic antitumor response after immunization with a recombinant adenovirus encoding a model tumor-associated antigen.Journal of immunology (Baltimore, Md. : 1950) · 1996Article
- Adaptation of nicotinic acetylcholine receptor, myogenin, and MRF4 gene expression to long-term muscle denervation.The Journal of cell biology · 1995Article
- Identification of a DNA element determining synaptic expression of the mouse acetylcholine receptor delta-subunit gene.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Acetylcholine receptor (AChR) genes are repressed in extrajunctional domains of adult muscle fiber by neurally evoked electrical activity. Denervation elicits upregulation of AChR gene transcription in extrasynaptic areas. We have used an adenovirus (Ad)-based strategy to analyze in vitro and in vivo the electrical activity-dependent transcription of the chicken AChR alpha 1 subunit gene. The luciferase gene placed under the control of wild-type and mutated fragments of the alpha 1 subunit promoter was inserted in a defective Ad vector designed for the study of transcriptional regulation. Animals were infected by intramuscular injection and in vivo luciferase levels were normalized by coinfection with an Ad vector containing the chloramphenicol acetyltransferase gene driven by an electrical activity-insensitive promoter. Our results demonstrate that although both proximal MyoD binding sites of the alpha 1 promoter are required for muscle-specific expression of the alpha 1 gene, only one is necessary, albeit insufficient, to enhance alpha 1 promoter activity after denervation. Parallel results were obtained with cultured muscle cells in vitro following tetrodotoxin blocking of spontaneous electrical activity. These results substantiate a direct contribution of MyoD factors in electrical activity-dependent regulation of AChR expression and further indicate that Ad-based vectors constitute a powerful tool in the field of transcriptional regulation.
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