ArticleProceedings of the National Academy of Sciences of the United States of America1994
Invariant tryptophan at a shielded site promotes folding of the conformational unit of spectrin.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 44 citations in OpenAlex.
- Multiple Functions of Spectrin: Convergent Effects.The Journal of membrane biology · 2020Review
- Alpha-actinin of the chlorarchiniophytePeerJ · 2018Article
- Computational method allowing Hydrogen-Deuterium Exchange Mass Spectrometry at single amide Resolution.Scientific reports · 2017Article
- Fluorescence study of the effect of cholesterol on spectrin-aminophospholipid interactions.European biophysics journal : EBJ · 2015Article
- Organization and dynamics of tryptophan residues in brain spectrin: novel insight into conformational flexibility.Journal of fluorescence · 2015Article
- Probing conformational stability and dynamics of erythroid and nonerythroid spectrin: effects of urea and guanidine hydrochloride.PloS one · 2015Article
- Structure and function of a spectrin-like regulator of bacterial cytokinesis.Nature communications · 2014Article
- Thermal stability of chicken brain α-spectrin repeat 17: a spectroscopic study.Journal of biomolecular NMR · 2012Article
- A Two-amino Acid Mutation Encountered in Duchenne Muscular Dystrophy Decreases Stability of the Rod Domain 23 (R23) Spectrin-like Repeat of Dystrophin.The Journal of biological chemistry · 2009Article
- Studying the folding of multidomain proteins.HFSP journal · 2008Article
- Structure and dynamics of de novo proteins from a designed superfamily of 4-helix bundles.Protein science : a publication of the Protein Society · 2008Article
- Structural analysis of the plakin domain of bullous pemphigoid antigen1 (BPAG1) suggests that plakins are members of the spectrin superfamily.Journal of molecular biology · 2007Article
- Hybrid spectrin type repeats produced by exon-skipping in dystrophin.Biochimica et biophysica acta · 2006Article
- Conformational study of spectrin in presence of submolar concentrations of denaturants.Journal of fluorescence · 2005Article
- Organization and dynamics of tryptophan residues in erythroid spectrin: novel structural features of denatured spectrin revealed by the wavelength-selective fluorescence approach.Protein science : a publication of the Protein Society · 2003Article
- Spectrin alpha II and beta II isoforms interact with high affinity at the tetramerization site.The Biochemical journal · 2003Article
- Spectrin self-association site: characterization and study of beta-spectrin mutations associated with hereditary elliptocytosis.The Biochemical journal · 1998Article
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The tryptophan that is highly conserved among repeating structural units of spectrin is reported to promote the conformational stability of one such unit of chicken brain alpha-spectrin. Four constructs were inserted into pET vectors for overexpression in Escherichia coli of the following spectrin peptides: (i) two adjacent but separately expressed "conformationally phased" repeating units, R16 and R17, one of which (R17) contains a single tryptophan; (ii) a mutant, M17, of the single tryptophan-containing unit with alanine substituted for the tryptophan; and (iii) a conformationally unphased unit, 1617, composed of half of each of the phased units. Both the mutant unit and the unphased unit were much more readily digested by chymotrypsin and by elastase than the phased units and exhibited only 38% and 54% as much alpha-helical structure, respectively, as the phased units by their far UV CD spectra; 90 degrees light scattering measurements revealed the folded peptides to be predominantly monomeric in solution, whereas the unfolded, protease-sensitive peptides consisted of dimers and/or trimers. This trend was corroborated by their dynamic light scattering. Both the blue-shifted wavelength of maximal emission and the relative inaccessibility to acrylamide of the single tryptophan in the folded unit indicate that the invariant tryptophan occupies a site that is shielded from the aqueous phase.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.