ArticleThe Journal of cell biology1994
Localization of the Kar3 kinesin heavy chain-related protein requires the Cik1 interacting protein.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 68 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
68 citing papers in PubMed, 121 citations in OpenAlex.
- Kar4, the yeast homolog of METTL14, is required for mRNA m6A methylation and meiosis.PLoS genetics · 2023Article
- Kar4, the Yeast Homolog of METTL14, is Required for mRNA mbioRxiv : the preprint server for biology · 2023Article
- Article
- Microtubule-associated proteins and motors required for ectopic microtubule array formation in Saccharomyces cerevisiae.Genetics · 2021Article
- Microtubule dynamics regulation reconstituted in budding yeast lysates.Journal of cell science · 2018Article
- The Aspergillus nidulans bimC4 mutation provides an excellent tool for identification of kinesin-14 inhibitors.Fungal genetics and biology : FG & B · 2015Article
- Candida albicans Kinesin Kar3 Depends on a Cik1-Like Regulatory Partner Protein for Its Roles in Mating, Cell Morphogenesis, and Bipolar Spindle Formation.Eukaryotic cell · 2015Article
- Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.Nature communications · 2015Article
- Minus-end-directed Kinesin-14 motors align antiparallel microtubules to control metaphase spindle length.Developmental cell · 2014Article
- Common mechanistic themes for the powerstroke of kinesin-14 motors.Journal of structural biology · 2013Article
- The molecular basis for kinesin functional specificity during mitosis.Cytoskeleton (Hoboken, N.J.) · 2013Review
- Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.Genes & development · 2013Article
- Functional asymmetry in kinesin and dynein dimers.Biology of the cell · 2013Review
- Article
- Neck rotation and neck mimic docking in the noncatalytic Kar3-associated protein Vik1.The Journal of biological chemistry · 2012Article
- The ATPase pathway that drives the kinesin-14 Kar3Vik1 powerstroke.The Journal of biological chemistry · 2012Article
- Kar3Vik1, a member of the kinesin-14 superfamily, shows a novel kinesin microtubule binding pattern.The Journal of cell biology · 2012Article
- Mitotic spindle form and function.Genetics · 2012Article
- Article
- Kinesin Kar3Cik1 ATPase pathway for microtubule cross-linking.The Journal of biological chemistry · 2011Article
8 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The Kar3 protein (Kar3p), a protein related to kinesin heavy chain, and the Cik1 protein (Cik1p) appear to participate in the same cellular processes in S. cerevisiae. Phenotypic analysis of mutants indicates that both CIK1 and KAR3 participate in spindle formation and karyogamy. In addition, the expression of both genes is induced by pheromone treatment. In vegetatively growing cells, both Cik1::beta-gal and Kar3::beta-gal fusions localize to the spindle pole body (SPB), and after pheromone treatment both fusion proteins localize to the spindle pole body and cytoplasmic microtubules. The dependence of Cik1p and Kar3p localization upon one another was investigated by indirect immunofluorescence of fusion proteins in pheromone-treated cells. The Cik1p::beta-gal fusion does not localize to the SPB or microtubules in a kar3 delta strain, and the Kar3p::beta-gal fusion protein does not localize to microtubule-associated structures in a cik1 delta strain. Thus, these proteins appear to be interdependent for localization to the SPB and microtubules. Analysis by both the two-hybrid system and co-immunoprecipitation experiments indicates that Cik1p and kar3p interact, suggesting that they are part of the same protein complex. These data indicate that interaction between a putative kinesin heavy chain-related protein and another protein can determine the localization of motor activity and thereby affect the functional specificity of the motor complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.