ArticleProceedings of the National Academy of Sciences of the United States of America1993
Neurotrophic activity of the Antennapedia homeodomain depends on its specific DNA-binding properties.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Exploring the Chemical Features and Biomedical Relevance of Cell-Penetrating Peptides.International journal of molecular sciences · 2024Review
- Influence of Aza-Glycine Substitution on the Internalization of Penetratin.Pharmaceutics · 2024Article
- In vivo direct cell-penetrating peptide mediated protein transduction system in Acyrthosiphon pisum.BMC research notes · 2023Article
- Aggregated Alpha-Synuclein Inclusions within the Nucleus Predict Impending Neuronal Cell Death in a Mouse Model of Parkinsonism.International journal of molecular sciences · 2022Article
- Designed Multifunctional Peptides for Intracellular Targets.Antibiotics (Basel, Switzerland) · 2022Review
- Control of brain patterning by Engrailed paracrine transfer: a new function of the Pbx interaction domain.Development (Cambridge, England) · 2015Article
- Review
- On guanidinium and cellular uptake.The Journal of organic chemistry · 2014Article
- Cell-penetrating peptides: achievements and challenges in application for cancer treatment.Journal of biomedical materials research. Part A · 2014Review
- Signaling with homeoprotein transcription factors in development and throughout adulthood.Current genomics · 2013Article
- Investigation of homeodomain membrane translocation properties: insights from the structure determination of engrailed-2 homeodomain in aqueous and membrane-mimetic environments.Biophysical journal · 2013Article
- Plant and animal homeodomains use convergent mechanisms for intercellular transfer.EMBO reports · 2005Article
- Penetratin-membrane association: W48/R52/W56 shield the peptide from the aqueous phase.Biophysical journal · 2005Article
- Charge-dependent translocation of the Trojan peptide penetratin across lipid membranes.Biophysical journal · 2003Article
- Cellular uptake of Antennapedia Penetratin peptides is a two-step process in which phase transfer precedes a tryptophan-dependent translocation.Nucleic acids research · 2003Article
- The identity and distribution of neural cells expressing the mesodermal determinant spadetail.BMC developmental biology · 2002Article
- Peptide delivery to the brain via adsorptive-mediated endocytosis: advances with SynB vectors.AAPS pharmSci · 2002Review
- The neuronal microtubule-associated protein 1B is under homeoprotein transcriptional control.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2001Article
- Protein transduction: an alternative to genetic intervention?Gene therapy · 2001Review
- Association study with two markers of a human homeogene in infantile autism.Journal of medical genetics · 1995Article
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5 authors.
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Abstract
In previous reports we have demonstrated that the 60-aa peptide corresponding to the homeodomain of the Drosophila protein Antennapedia (pAntp) translocates through the membrane of neurons in culture, accumulates in neuronal nuclei, and promotes neurite growth. To analyze the importance of specific pAntp DNA-binding properties in this phenomenon we have constructed three mutant versions of pAntp that differ in their ability to translocate through the membrane and to bind specifically the cognate sequence for homeodomains present in the promoter of HoxA5. We demonstrate that removing two hydrophobic residues of the third helix inhibits pAntp internalization and suppresses its neurotrophic activity. We also show that pAntp neurotrophic activity is lost when mutations are introduced in positions preserving its penetration and nuclear accumulation but abolishing its capacity to bind specifically the cognate DNA-binding motif for homeoproteins. Our results strongly suggest that pAntp neurotrophicity requires both its internalization and its specific binding to homeobox cognate sequences. We propose that homeoproteins might regulate important events in the morphological differentiation of the postmitotic neuron.
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