ArticleNucleic acids research1993
Cloning and regulation of the rat mdr2 gene.
Article in Nucleic acids research, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 79 citations in OpenAlex.
- Peroxisome proliferator-activated receptor α activates human multidrug resistance transporter 3/ATP-binding cassette protein subfamily B4 transcription and increases rat biliary phosphatidylcholine secretion.Hepatology (Baltimore, Md.) · 2014Article
- Stereoselective transport of hydrophilic quaternary drugs by human MDR1 and rat Mdr1b P-glycoproteins.British journal of pharmacology · 2002Article
- Physiological oxygen tensions modulate expression of the mdr1b multidrug-resistance gene in primary rat hepatocyte cultures.The Biochemical journal · 2000Article
- Regulation and expression of multidrug resistance (MDR) transcripts in the intestinal epithelium.British journal of cancer · 1999Article
- Selective inhibition of MDR1 P-glycoprotein-mediated transport by the acridone carboxamide derivative GG918.British journal of cancer · 1999Article
- Molecular analysis of the multidrug transporter, P-glycoprotein.Cytotechnology · 1998Article
- Decreased expression and activity of P-glycoprotein in rat liver during acute inflammation.Pharmaceutical research · 1998Article
- Characterization of the rat mdr2 promoter and its regulation by the transcription factor Sp1.Nucleic acids research · 1996Article
- Localization of rat Pgy3 (mdr2) to the same region as Pgy2 (mdr1b) at 4q11-12.Mammalian genome : official journal of the International Mammalian Genome Society · 1996Article
- Regulation of P-glycoprotein 1 and 2 gene expression and protein activity in two MCF-7/Dox cell line subclones.British journal of cancer · 1996Article
- P-glycoprotein, multidrug resistance and tumor progression.Cancer metastasis reviews · 1994Review
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have cloned the complete cDNA encoding the rat mdr2 gene by a combination of library screening and the polymerase chain reaction. The sequence of rat mdr2 cDNA is highly similar to other members of the mdr gene family but the initiation of transcription, tissue distribution and regulation of expression of rat mdr2 diverge from the other isoforms. Primer extension analysis showed rat mdr2 mRNA to have a major transcription start point at -277 and a minor one at approximately -518. We constructed gene specific probes for rat mdr2 and mdr1b and compared the expression patterns of these two genes. The highest expression of mdr2 mRNA was in the muscle, heart, liver and spleen. Both mdr2 and 1b mRNA levels were elevated in the livers of rats treated with CCl4 or following partial hepatectomies although the time course of induction of each gene differed. Mdr1b increased by 12 to 24 hours while mdr2 did not increase until 48 hours. Treatment of isolated hepatocytes or RC3 cells with cycloheximide did not effect mdr2 mRNA. In contrast, mdr1b expression was increased. These data suggest that rat mdr2, unlike mdr1b, is not regulated by a negative trans-acting protein factor.
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