ArticlePlant molecular biology1993
Molecular characterization of a cDNA clone encoding glutamine synthetase from a gymnosperm, Pinus sylvestris.
Article in Plant molecular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 44 citations in OpenAlex.
- A revised view on the evolution of glutamine synthetase isoenzymes in plants.The Plant journal : for cell and molecular biology · 2022Article
- Low assimilation efficiency of photorespiratory ammonia in conifer leaves.Journal of plant research · 2018Article
- Glutamate synthases from conifers: gene structure and phylogenetic studies.BMC genomics · 2018Article
- Article
- In vitro promoter recognition by the catalytic subunit of plant phage-type RNA polymerases.Plant molecular biology · 2016Article
- Article
- Molecular aspects of nitrogen mobilization and recycling in trees.Photosynthesis research · 2005Review
- Article
- Both phyA and phyB mediate light-imposed repression of PHYA gene expression in Arabidopsis.Plant physiology · 1999Article
- Expression analysis of a cytosolic glutamine synthetase gene in cotyledons of Scots pine seedlings: developmental, light regulation and spatial distribution of specific transcripts.Plant molecular biology · 1999Article
- Characterization of Vitis vinifera L. glutamine synthetase and molecular cloning of cDNAs for the cytosolic enzyme.Plant molecular biology · 1996Article
- Expression of ferredoxin-dependent glutamate synthase in dark-grown pine seedlings.Plant molecular biology · 1995Article
- Biphasic and differential expression of cytosolic glutamine synthetase genes of radish during seed germination and senescence of cotyledons.Plant molecular biology · 1994Article
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5 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
A full-length cDNA clone (pGSP114) encoding glutamine synthetase was isolated from a lambda gt11 library of the gymnosperm Pinus sylvestris. Nucleotide sequence analysis showed that pGSP114 contains an open reading frame encoding a protein of 357 amino acid residues with a calculated molecular mass of 39.5 kDa. The derived amino acid sequence was more homologous to cytosolic (GS1) (78-82%) than to chloroplastic (GS2) (71-75%) glutamine synthetase in angiosperms. The lack of N-terminal presequence and C-terminal extension which define the primary structure of GS2, also supports that the isolated cDNA encodes cytosolic GS. Southern blot analysis of genomic DNA from P. sylvestris and P. pinaster suggests that GS may be encoded by a small gene family in pine. GS mRNA was more abundant in cotyledons and stems than in roots of both Scots and maritime pines. Western blot analysis in P. sylvestris seedlings showed that only one GS polypeptide, similar in size to GS1 in P. pinaster, could be detected in several different tissues. Our results suggest that cytosolic GS is mainly responsible for glutamine biosynthesis in pine seedlings.
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