ArticleMolecular and cellular biology1993
Direct transcriptional stimulation of the ornithine decarboxylase gene by Fos in PC12 cells but not in fibroblasts.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 54 citations in OpenAlex.
- Transcriptome profiling of maternal stress-induced wing dimorphism in pea aphids.Ecology and evolution · 2019Article
- Role of Polyamines in Immune Cell Functions.Medical sciences (Basel, Switzerland) · 2018Review
- A perspective of polyamine metabolism.The Biochemical journal · 2003Review
- Myc drives apoptosis in PC12 cells in the absence of Max.Oncogene · 2001Article
- ODC mRNA as a prognostic factor for predicting recurrence in meningiomas.Journal of neuro-oncology · 2001Article
- Complementation of defective colony-stimulating factor 1 receptor signaling and mitogenesis by Raf and v-Src.Molecular and cellular biology · 1999Article
- Anti-tumor activity of antizyme which targets the ornithine decarboxylase (ODC) required for cell growth and transformation.Oncogene · 1999Article
- Inhibition of the expression of ornithine decarboxylase and c-Myc by cell-permeant ceramide in difluoromethylornithine-resistant leukaemia cells.The Biochemical journal · 1997Article
- Transcription factor Sp3 antagonizes activation of the ornithine decarboxylase promoter by Sp1.Nucleic acids research · 1997Article
- The five cleavage-stage (CS) histones of the sea urchin are encoded by a maternally expressed family of replacement histone genes: functional equivalence of the CS H1 and frog H1M (B4) proteins.Molecular and cellular biology · 1997Article
- Rodent cell transformation and immediate early gene expression following 60-Hz magnetic field exposure.Environmental health perspectives · 1996Article
- Characterization of cell-specific modulatory element in the murine ornithine decarboxylase promoter.The Biochemical journal · 1996Article
- Ornithine decarboxylase- and ras-induced cell transformations: reversal by protein tyrosine kinase inhibitors and role of pp130CAS.Molecular and cellular biology · 1995Article
- Transcriptional activation of the fra-1 gene by AP-1 is mediated by regulatory sequences in the first intron.Molecular and cellular biology · 1995Article
- Cell transformation by c-fos requires an extended period of expression and is independent of the cell cycle.Molecular and cellular biology · 1994Article
- Alternative promoter usage of the Fos-responsive gene Fit-1 generates mRNA isoforms coding for either secreted or membrane-bound proteins related to the IL-1 receptor.The EMBO journal · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have established rat PC12 pheochromocytoma cell lines stably expressing the estrogen-activatable transcription factor FosER to identify genes that can be regulated by c-Fos in this neuronal cell type. Induction of ectopic c-Fos activity in PC12 cells increased the mRNA levels of the ornithine decarboxylase (ODC) and tyrosine hydroxylase genes with similar kinetics and to the same maximal level as nerve growth factor treatment. In both cases the rate of transcription initiation was increased. Induction of the ODC gene occurred even in the absence of protein synthesis, indicating direct regulation by FosER. ODC expression, however, was not induced by a mutant FosER protein containing a proline insertion in the basic region of the c-Fos moiety, demonstrating the requirement for a functional DNA-binding domain. These data show that FosER, and by extrapolation c-Fos, can directly activate transcription of the endogenous ODC gene in PC12 cells by binding to cis-regulatory sequences. Activation of the ODC gene was unexpectedly transient, as transcripts returned to the basal level after prolonged exposure of PC12 cells to FosER activity. Furthermore, ODC transcription was not at all induced by FosER in rat fibroblasts. To account for this cell-specific action of FosER, we propose that stimulation of the ODC gene by FosER requires either (i) cooperation with another transcription factor(s) or (ii) a specific pattern of modification which is present in PC12 cells but not in otherwise unstimulated fibroblasts. One or both of these mechanisms may be employed by cells to achieve selective gene activation in response to apparently stereotyped induction of c-fos.
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