ArticleJournal of clinical microbiology1993
Mixed-linker polymerase chain reaction: a new method for rapid fingerprinting of isolates of the Mycobacterium tuberculosis complex.
Article in Journal of clinical microbiology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 49 papers.
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Who cites it
49 citing papers in PubMed.
- Automated IS6110-based fingerprinting of Mycobacterium tuberculosis: Reaching unprecedented discriminatory power and versatility.PloS one · 2018Article
- Methodological and Clinical Aspects of the Molecular Epidemiology of Mycobacterium tuberculosis and Other Mycobacteria.Clinical microbiology reviews · 2016Review
- Comparison of ligation-mediated PCR methods in differentiation of Mycobacterium tuberculosis strains.BioMed research international · 2014Article
- Current methods in the molecular typing of Mycobacterium tuberculosis and other mycobacteria.BioMed research international · 2014Review
- Genotyping of clinical Mycobacterium tuberculosis isolates based on IS6110 and MIRU-VNTR polymorphisms.BioMed research international · 2013Article
- Evolution of the mycobacterial SigK regulon.Journal of bacteriology · 2008Article
- Discriminatory power and reproducibility of novel DNA typing methods for Mycobacterium tuberculosis complex strains.Journal of clinical microbiology · 2005Article
- Fast ligation-mediated PCR, a fast and reliable method for IS6110-based typing of Mycobacterium tuberculosis complex.Journal of clinical microbiology · 2005Article
- Polymorphism of variable-number tandem repeats at multiple loci in Mycobacterium tuberculosis.Journal of clinical microbiology · 2005Article
- Use of the hupB gene encoding a histone-like protein of Mycobacterium tuberculosis as a target for detection and differentiation of M. tuberculosis and M. bovis.Journal of clinical microbiology · 2004Article
- Detection and identification of Mycobacterium species isolates by DNA microarray.Journal of clinical microbiology · 2003Article
- Improvement of differentiation and interpretability of spoligotyping for Mycobacterium tuberculosis complex isolates by introduction of new spacer oligonucleotides.Journal of clinical microbiology · 2002Article
- Evaluation of the Mycobacterium bovis restriction fragment length polymorphism probe pUCD, in combination with the direct repeat probe, for molecular typing of Mycobacterium tuberculosis strains in Ireland.Journal of clinical microbiology · 2001Article
- Reverse dot blot assay (insertion site typing) for precise detection of sites of IS6110 insertion in the Mycobacterium tuberculosis genome.Journal of clinical microbiology · 2001Article
- Review
- Mapping of IS6110 insertion sites in two epidemic strains of Mycobacterium tuberculosis.Journal of clinical microbiology · 2000Article
- Simultaneous infection with two drug-susceptible Mycobacterium tuberculosis strains in an immunocompetent host.Journal of clinical microbiology · 1999Article
- Transmission dynamics of tuberculosis in a high-incidence country: prospective analysis by PCR DNA fingerprinting.Journal of clinical microbiology · 1999Article
- Comparative evaluation of ligation-mediated PCR and spoligotyping as screening methods for genotyping of Mycobacterium tuberculosis strains.Journal of clinical microbiology · 1999Article
- Amplified-fragment length polymorphism analysis: the state of an art.Journal of clinical microbiology · 1999Review
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4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rapid recognition of multidrug-resistant strains of Mycobacterium tuberculosis is a desirable goal for treatment of patients and protection of health care workers. DNA fingerprints produced with the insertion sequence IS6110 generate restriction fragment length polymorphism (RFLP) patterns that reliably identify M. tuberculosis complex strains. This report describes a rapid technique for RFLP typing using the polymerase chain reaction. The method uses one primer specific for IS6110 and a second primer complementary to a linker ligated to the restricted genomic DNA. In one strand the linker contains uracil in place of thymidine, and specific amplification is obtained by elimination of this strand with uracil N-glycosylase. Mixed-linker fingerprinting clearly differentiated multidrug-resistant isolates from 12 outbreaks and unambiguously assigned them to 26 RFLP groups.
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