ArticleThe Journal of cell biology1993
The pas8 mutant of Pichia pastoris exhibits the peroxisomal protein import deficiencies of Zellweger syndrome cells--the PAS8 protein binds to the COOH-terminal tripeptide peroxisomal targeting signal, and is a member of the TPR protein family.
Article in The Journal of cell biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- Structural basis of receptor retro-translocation in peroxisomal protein import.bioRxiv : the preprint server for biology · 2026Article
- PEX14 condensates recruit receptor and cargo pairs for peroxisomal protein import.Nature structural & molecular biology · 2025Article
- A Mechanistic Perspective on PEX1 and PEX6, Two AAA+ Proteins of the Peroxisomal Protein Import Machinery.International journal of molecular sciences · 2019Review
- Peroxisome protein import recapitulated inThe Journal of cell biology · 2019Article
- The Principles of Protein Targeting and Transport Across Cell Membranes.The protein journal · 2019Review
- Current Advances in Protein Import into Peroxisomes.The protein journal · 2019Review
- Article
- Peroxisomal monoubiquitinated PEX5 interacts with the AAA ATPases PEX1 and PEX6 and is unfolded during its dislocation into the cytosol.The Journal of biological chemistry · 2018Article
- The PEX1 ATPase Stabilizes PEX6 and Plays Essential Roles in Peroxisome Biology.Plant physiology · 2017Article
- Identification and characterization of the peroxin 1 gene MoPEX1 required for infection-related morphogenesis and pathogenicity in Magnaporthe oryzae.Scientific reports · 2016Article
- Evolutionary loss of peroxisomes--not limited to parasites.Biology direct · 2015Article
- Revisiting the intraperoxisomal pathway of mammalian PEX7.Scientific reports · 2015Article
- Dynein light chain interaction with the peroxisomal import docking complex modulates peroxisome biogenesis in yeast.Journal of cell science · 2013Article
- Disrupting autophagy restores peroxisome function to an Arabidopsis lon2 mutant and reveals a role for the LON2 protease in peroxisomal matrix protein degradation.The Plant cell · 2013Article
- Redox-regulated cargo binding and release by the peroxisomal targeting signal receptor, Pex5.The Journal of biological chemistry · 2013Article
- Atg35, a micropexophagy-specific protein that regulates micropexophagic apparatus formation in Pichia pastoris.Autophagy · 2011Article
- Controlling subcellular delivery to optimize therapeutic effect.Therapeutic delivery · 2010Review
- Regulation of peroxisome dynamics.Current opinion in cell biology · 2009Review
- Protein quaternary structure and expression levels contribute to peroxisomal-targeting-sequence-1-mediated peroxisomal import of human soluble epoxide hydrolase.Journal of molecular biology · 2008Article
- Two independent pathways traffic the intraperoxisomal peroxin PpPex8p into peroxisomes: mechanism and evolutionary implications.Molecular biology of the cell · 2006Article
22 more citing papers are in PubMed but not listed here.
Corrections and comments
- Erratum issued
Authors and funding
3 authors.
Funding
Abstract
We previously described the isolation of mutants of the yeast Pichia pastoris that are deficient in peroxisome assembly (pas mutants). We describe the characterization of one of these mutants, pas8, and the cloning of the PAS8 gene. The pas8 mutant is deficient for growth, but not for division or segregation of peroxisomes, or for induction of peroxisomal proteins. Two distinct peroxisomal targeting signals, PTS1 and PTS2, have been identified that are sufficient to direct proteins to the peroxisomal matrix. We show that the pas8 mutant is deficient in the import of proteins with the PTS1, but not the PTS2, targeting signal. This is the same import deficiency as that found in cells from patients with the lethal human peroxisomal disorder Zellweger syndrome. Cloning and sequencing of the PAS8 gene reveals that it is a novel member of the tetratricopeptide repeat gene family. Antibodies raised against bacterially expressed PAS8 are used to show that PAS8 is a peroxisomal, membrane-associated protein. Also, we have found that in vitro translated PAS8 protein is capable of binding the PTS1 targeting signal specifically, raising the possibility that PAS8 is a PTS1 receptor.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.