ArticleThe Journal of clinical investigation1993
Characterization and distribution of alpha 2-adrenergic receptors in the human intestinal mucosa.
Article in The Journal of clinical investigation, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
24 citing papers in PubMed, 59 citations in OpenAlex.
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- Serum amyloid A expression in liver promotes synovial macrophage activation and chronic arthritis via NFAT5.The Journal of clinical investigation · 2024Article
- Sympathetic activity regulates epithelial proliferation and wound healing via adrenergic receptor αScientific reports · 2023Article
- Identification of a neural development gene expression signature in colon cancer stem cells reveals a role foriScience · 2022Article
- Effects of α2-adrenoceptor stimulation on luminal alkalinisation and net fluid flux in rat duodenum.PloS one · 2022Article
- The Impact of α-Adrenoceptors in the Regulation of the Hypotonicity-Induced Increase in Duodenal Mucosal Permeability In Vivo.Pharmaceutics · 2021Article
- A link between gastrointestinal disorders and migraine: Insights into the gut-brain connection.Headache · 2021Review
- Regulation of the Autonomic Nervous System on Intestine.Frontiers in physiology · 2021Review
- Biogenic Amines: Signals Between Commensal Microbiota and Gut Physiology.Frontiers in endocrinology · 2019Article
- In situ-forming and pH-responsive hydrogel based on chitosan for vaginal delivery of therapeutic agents.Journal of materials science. Materials in medicine · 2018Article
- Evidence for a direct effect of the autonomic nervous system on intestinal epithelial stem cell proliferation.Physiological reports · 2018Article
- A complex relationship: the interaction among symbiotic microbes, invading pathogens, and their mammalian host.Mucosal immunology · 2011Review
- Regulation of electroneutral NaCl absorption by the small intestine.Annual review of physiology · 2011Review
- Investigation of the distribution and function of alpha-adrenoceptors in the sheep isolated internal anal sphincter.British journal of pharmacology · 2010Article
- Alpha2-adrenergic receptors attenuate secretagogue-induced endocytosis and promote exocytosis of intestinal NHE2 and NHE3.The Journal of pharmacology and experimental therapeutics · 2009Article
- Adrenergic activation of electrogenic K+ secretion in guinea pig distal colonic epithelium: involvement of beta1- and beta2-adrenergic receptors.American journal of physiology. Gastrointestinal and liver physiology · 2009Article
- Principles and clinical implications of the brain-gut-enteric microbiota axis.Nature reviews. Gastroenterology & hepatology · 2009Review
- Article
- Inter-kingdom signalling: communication between bacteria and their hosts.Nature reviews. Microbiology · 2008Review
- Mucosally-directed adrenergic nerves and sympathomimetic drugs enhance non-intimate adherence of Escherichia coli O157:H7 to porcine cecum and colon.European journal of pharmacology · 2006Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The subtype and the expression of the alpha 2-adrenergic receptor were investigated in the normal mucosa from human intestine by means of radioligand binding, RNase mapping, and measurement of adenylate cyclase activity. The study of the binding of the alpha 2-adrenergic antagonist, [3H]RX821002, to epithelial cell membranes indicated the existence of a single class of noninteracting sites displaying a high affinity for the radioligand (Kd = 1.1 +/- 0.5 nM). The rank order of potency of antagonists to inhibit [3H]RX821002 binding (RX821002 > yohimbine = rauwolscine > phentolamine approximately idazoxan >> chlorpromazine > prazosin) suggested that the receptor is of the alpha 2A subtype. A conclusion which is confirmed by the fact that only alpha 2C10 transcripts were found in the human intestine mucosa. Competition curves with (-)-norepinephrine demonstrated that 60% of the receptor population exhibited high affinity for agonists. This high-affinity state was abolished by the addition of GTP plus Na+ or by prior treatment of the membranes with pertussis toxin indicating it corresponded to G protein-coupled receptors. [32P]ADP-ribosylation and immunoblotting experiments identified two pertussis toxin-sensitive G proteins corresponding to Gi2 and Gi3. The study of the distribution of the receptor indicated that (a) the proximal colon is the intestine segment exhibiting the highest receptor density and (b) the receptor is predominantly expressed in crypts and is preferentially located in the basolateral membrane of the polarized cell. The distribution of the receptor along the crypt-surface axis of the colon mucosa can be correlated with a higher level of alpha 2C10-specific mRNA and a higher efficiency of UK14304 to inhibit adenylate cyclase in crypt cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.