ArticleMolecular & general genetics : MGG1993
The Neurospora uvs-2 gene encodes a protein which has homology to yeast RAD18, with unique zinc finger motifs.
Article in Molecular & general genetics : MGG, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
13 citing papers in PubMed, 41 citations in OpenAlex.
- A novel transcription factor gene FHS1 is involved in the DNA damage response in Fusarium graminearum.Scientific reports · 2016Article
- Neurosprora crassa RAD5 homologue, mus-41, inactivation results in higher sensitivity to mutagens but has little effect on PCNA-ubiquitylation in response to UV-irradiation.Current genetics · 2007Article
- A high-throughput gene knockout procedure for Neurospora reveals functions for multiple transcription factors.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Srs2 and RecQ homologs cooperate in mei-3-mediated homologous recombination repair of Neurospora crassa.Nucleic acids research · 2005Article
- Highly efficient gene replacements in Neurospora strains deficient for nonhomologous end-joining.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.Microbiology and molecular biology reviews : MMBR · 2004Review
- The Neurospora crassa mus-19 gene is identical to the qde-3 gene, which encodes a RecQ homologue and is involved in recombination repair and postreplication repair.Current genetics · 2004Article
- Enhanced genomic instability and defective postreplication repair in RAD18 knockout mouse embryonic stem cells.Molecular and cellular biology · 2003Article
- The human RAD18 gene product interacts with HHR6A and HHR6B.Nucleic acids research · 2000Article
- Dysfunction of human Rad18 results in defective postreplication repair and hypersensitivity to multiple mutagens.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- Identification and expression of the Neurospora crassa mei-3 gene which encodes a protein homologous to Rad51 of Saccharomyces cerevisiae.Molecular & general genetics : MGG · 1995Article
- The Aspergillus uvsH gene encodes a product homologous to yeast RAD18 and Neurospora UVS-2.Molecular & general genetics : MGG · 1995Article
- uvsI mutants defective in UV mutagenesis define a fourth epistatic group of uvs genes in Aspergillus.Current geneticsArticle
Corrections and comments
- Erratum issued
Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A clone containing the DNA repair gene uvs-2 of Neurospora crassa was identified from a Neurospora genomic DNA library using the sib-selection method. Transformants were screened for resistance to methyl methane sulfonate (MMS). A DNA fragment that complements the uvs-2 mutation was subcloned by monitoring its ability to transform the uvs-2 mutant to MMS resistance. Deletion analysis of the cloned DNA indicated that the size of the uvs-2 gene is approximately 1.6 kb. The identity of the uvs-2 gene was verified by restriction fragment length polymorphism (RFLP) mapping. The sensitivity of the transformant to three different mutagens was similar to that of the wild-type strain. Nucleotide sequences of genomic DNA and cDNA of the uvs-2 gene indicated that it has an open reading frame (ORF) of 1572 bp with a 69 bp intron in the middle of the sequence. Two transcription initiation sites located around 73 bp and 290 bp upstream of the translation initiation codon were identified by primer extension experiments. Northern analysis revealed that the nature transcript of the uvs-2 gene was about 1.8 kb long. The uvs-2 gene ORF is deduced to encode a polypeptide of 501 amino acids with a molecular mass of 54 kDa. The proposed polypeptide has 25% identity to the RAD18 polypeptide of Saccharomyces cerevisiae and contains two unique zinc finger motifs for nucleic acid binding. Similarities between the phenotypes of the rad18 and uvs-2 mutants suggest that the uvs-2 gene encodes a protein which is involved in postreplication repair, rather than excision repair.
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