ArticleMolecular and cellular biology1993
A complex promoter element mediates transactivation of the human proliferating cell nuclear antigen promoter by the 243-residue adenovirus E1A oncoprotein.
Article in Molecular and cellular biology, 1993. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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14 citing papers in PubMed, 32 citations in OpenAlex.
- Two estrogen response element sequences near the PCNA gene are not responsible for its estrogen-enhanced expression in MCF7 cells.PloS one · 2008Article
- Article
- p53-mediated regulation of proliferating cell nuclear antigen expression in cells exposed to ionizing radiation.Molecular and cellular biology · 1999Article
- A cellular repressor of E1A-stimulated genes that inhibits activation by E2F.Molecular and cellular biology · 1998Article
- Regulation of the human proliferating cell nuclear antigen promoter by the adenovirus E1A-associated protein p107.Journal of virology · 1998Article
- Transcriptional coactivator cAMP response element binding protein mediates induction of the human proliferating cell nuclear antigen promoter by the adenovirus E1A oncoprotein.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Human T-cell leukemia virus type 1 Tax transactivates the human proliferating cell nuclear antigen promoter.Journal of virology · 1997Article
- The CtBP binding domain in the adenovirus E1A protein controls CR1-dependent transactivation.Nucleic acids research · 1996Article
- Transcriptional activation of the human proliferating-cell nuclear antigen promoter by p53.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Transcription factors RFX1/EF-C and ATF-1 associate with the adenovirus E1A-responsive element of the human proliferating cell nuclear antigen promoter.Nucleic acids research · 1995Article
- Regulation of cyclin D1, DNA topoisomerase I, and proliferating cell nuclear antigen promoters during the cell cycle.Gene expression · 1995Article
- An adenovirus E1A transcriptional repressor domain functions as an activator when tethered to a promoter.Nucleic acids research · 1994Article
- Promoter activity of the proliferating-cell nuclear antigen gene is associated with inducible CRE-binding proteins in interleukin 2-stimulated T lymphocytes.Molecular and cellular biology · 1994Article
- Modulation of transcriptional activation of the proliferating cell nuclear antigen promoter by the adenovirus E1A 243-residue oncoprotein depends on proximal activators.Molecular and cellular biology · 1994Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
The adenovirus E1A oncoproteins interfere with the normal regulation of cellular proliferation through interactions with cell cycle regulatory proteins. In view of the essential role of proliferating-cell nuclear antigen (PCNA) in DNA replication, we performed a mutational analysis of the minimal human PCNA promoter (nucleotides -87 to +62) to define sequence elements which mediate transactivation by the 243-residue E1A protein (E1A 243R). Linker-scanning and site-directed mutants were examined for basal and E1A-induced expression of chloramphenicol acetyltransferase (CAT) from PCNA promoter-CAT reporter constructs transiently expressed in HeLa cells. The results define the cis-acting element required for induction of PCNA by E1A 243R as a region between -59 and -45 relative to the transcription initiation site. This PCNA E1A-responsive element (PERE), which is protected from DNase I digestion by nuclear extracts from 293 cells, includes the sequence AGCGTGG immediately upstream of the ATF binding site previously shown to be important for activation of PCNA by E1A 243R (G. F. Morris and M. B. Mathews, J. Virol. 65:6397-6406, 1991). Mutation of either the upstream component or the ATF site within the PERE diminishes basal promoter activity and abrogates transactivation by E1A 243R. This novel cis-acting element is also essential for both basal and E1A-induced expression in the context of the full-length PCNA promoter.
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