ArticleThe Biochemical journal1994
Phosphatidylinositol 3-kinase binds to alpha-actinin through the p85 subunit.
Article in The Biochemical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 23 papers.
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Who cites it
23 citing papers in PubMed, 92 citations in OpenAlex.
- α-Actinin1 promotes tumorigenesis and epithelial-mesenchymal transition of gastric cancer via the AKT/GSK3β/β-Catenin pathway.Bioengineered · 2021Article
- Article
- Class IA PI3K regulatory subunits: p110-independent roles and structures.Biochemical Society transactions · 2020Review
- The fifth sense: Mechanosensory regulation of alpha-actinin-4 and its relevance for cancer metastasis.Seminars in cell & developmental biology · 2017Review
- ESCRT-0 Component Hrs Promotes Macropinocytosis of Kaposi's Sarcoma-Associated Herpesvirus in Human Dermal Microvascular Endothelial Cells.Journal of virology · 2016Article
- The biological role of actinin-4 (ACTN4) in malignant phenotypes of cancer.Cell & bioscience · 2015Article
- Alpha-actinin 4 and tumorigenesis of breast cancer.Vitamins and hormones · 2013Review
- A genome scan for positive selection in thoroughbred horses.PloS one · 2009Article
- Cadherins are regulated by Ep-CAM via phosphaditylinositol-3 kinase.Molecular and cellular biochemistry · 2007Article
- A retrovirus-based protein complementation assay screen reveals functional AKT1-binding partners.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Protein-lipid interactions: correlation of a predictive algorithm for lipid-binding sites with three-dimensional structural data.Theoretical biology & medical modelling · 2006Article
- The integrin-actin connection, an eternal love affair.The EMBO journal · 2003Review
- Metalloprotease-disintegrin ADAM 12 interacts with alpha-actinin-1.The Biochemical journal · 2001Article
- Phosphatidylinositol 3' kinase signaling in mammary tumorigenesis.Journal of mammary gland biology and neoplasia · 2001Review
- The interaction of titin and alpha-actinin is controlled by a phospholipid-regulated intramolecular pseudoligand mechanism.The EMBO journal · 2000Article
- Meltrin alpha cytoplasmic domain interacts with SH3 domains of Src and Grb2 and is phosphorylated by v-Src.Oncogene · 2000Article
- Talin contains three similar vinculin-binding sites predicted to form an amphipathic helix.The Biochemical journal · 1999Article
- Phosphoinositide 3-kinase: the key switch mechanism in insulin signalling.The Biochemical journal · 1998Review
- Tyrosine phosphorylation-dependent activation of phosphatidylinositide 3-kinase occurs upstream of Ca2+-signalling induced by Fcgamma receptor cross-linking in human neutrophils.The Biochemical journal · 1997Article
- Activation of the osmo-sensitive chloride conductance involves P21rho and is accompanied by a transient reorganization of the F-actin cytoskeleton.Molecular biology of the cell · 1996Article
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphatidylinositol 3-kinase (PI 3-kinase) has been shown to play an important role in the signal transduction of cell growth. It is also suggested that it is involved in cytoskeletal reorganization. We have found that alpha-actinin copurifies with PI 3-kinase from bovine thymus. The antibody against PI 3-kinase 85 kDa subunit (p85) also co-immunoprecipitates alpha-actinin from lysates of NIH/3T3 cells. In addition, anti-alpha-actinin antibody coprecipitates PI 3-kinase activity. This coprecipitation was observed even after depolymerization of actin fibres, suggesting that PI 3-kinase binds directly to alpha-actinin. As alpha-actinin is a phosphatidylinositol 4,5-bisphosphate (PI4,5P2)-binding protein, binding experiments using various constructs of truncated p85 were carried out in the presence or absence of PI4,5P2. In the absence of PI4,5P2, chicken gizzard alpha-actinin binds only to the whole p85 construct, but it binds to the proline-rich region of p85 fragments in the presence of PI4,5P2. This binding is enhanced with increased concentrations of Pi4,5P2 up to 10 microM, whereas phosphatidylinositol and phosphatidylinositol 4-phosphate were not good activators of alpha-actinin binding. These results suggest that PI 3-kinase binds to alpha-actinin and regulates cytoskeletal reorganization.
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