ReviewAntonie van Leeuwenhoek
The yeast Kluyveromyces lactis as an efficient host for heterologous gene expression.
Review in Antonie van Leeuwenhoek. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed.
- Fast-track development of a lactase production process withEngineering in life sciences · 2017Article
- Cloning, production, and functional expression of the bacteriocin enterocin A, produced by Enterococcus faecium T136, by the yeasts Pichia pastoris, Kluyveromyces lactis, Hansenula polymorpha, and Arxula adeninivorans.Applied and environmental microbiology · 2012Article
- High-throughput screening and selection of yeast cell lines expressing monoclonal antibodies.Journal of industrial microbiology & biotechnology · 2010Article
- Acetamide selection of Kluyveromyces lactis cells transformed with an integrative vector leads to high-frequency formation of multicopy strains.Applied and environmental microbiology · 2007Article
- Induction by hypoxia of heterologous-protein production with the KlPDC1 promoter in yeasts.Applied and environmental microbiology · 2007Article
- Kluyveromyces lactis LAC4 promoter variants that lack function in bacteria but retain full function in K. lactis.Applied and environmental microbiology · 2005Article
- Improved production of heterologous proteins by a glucose repression-defective mutant of Kluyveromyces lactis.Applied and environmental microbiology · 2004Article
- Evolution, biochemistry and genetics of protein kinase C in fungi.Current genetics · 2003Review
- Use of the KlADH4 promoter for ethanol-dependent production of recombinant human serum albumin in Kluyveromyces lactis.Applied and environmental microbiology · 1999Article
- Heterologous protein secretion directed by a repressible acid phosphatase system of Kluyveromyces lactis: characterization of upstream region-activating sequences in the KIPHO5 gene.Applied and environmental microbiology · 1998Article
- Expression and secretion of recombinant ovine beta-lactoglobulin in Saccharomyces cerevisiae and Kluyveromyces lactis.The Biochemical journal · 1996Article
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several different yeast species have been developed into systems for efficient heterologous gene expression. In this paper we review foreign gene expression in the dairy yeast Kluyveromyces lactis. This yeast presents several advantageous properties in comparison to other yeast species. These include its impressive secretory capacities, its excellent fermentation characteristics on large scale, its food grade status and the availability of both episomal and integrative expression vectors. Moreover, in contrast to the methylotrophic yeasts that are frequently used for the expression of foreign genes, K. lactis does not require explosion-proof fermentation equipment. Here, we present an overview of the available tools for heterologous gene expression in K. lactis (available promoters, vector systems, etc). Also, the production of prochymosin, human serum albumin and pancreatic phospholipase by K. lactis is discussed in more detail, and used to rate the achievements of K. lactis with respect to other micro-organisms in which these proteins have been produced.
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