ArticleProceedings of the National Academy of Sciences of the United States of America1994
Interaction between the aphid transmission factor and virus particles is a part of the molecular mechanism of cauliflower mosaic virus aphid transmission.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
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Who cites it
11 citing papers in PubMed, 45 citations in OpenAlex.
- Identification and Genome Characterization of a Dahlia Common Mosaic Virus Isolate from China.Genes · 2023Article
- The Identification of Viral Pathogens in aViruses · 2023Article
- Enhancing Capsid Proteins Capacity in Plant Virus-Vector Interactions and Virus Transmission.Cells · 2021Review
- Route of a Multipartite Nanovirus across the Body of Its Aphid Vector.Journal of virology · 2020Article
- A variant of Rubus yellow net virus with altered genomic organization.Virus genes · 2015Article
- A single amino acid position in the helper component of cauliflower mosaic virus can change the spectrum of transmitting vector species.Journal of virology · 2005Article
- Intracellular distribution of viral gene products regulates a complex mechanism of cauliflower mosaic virus acquisition by its aphid vector.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Biochemical characterization of the helper component of Cauliflower mosaic virus.Journal of virology · 2001Article
- Interaction between the open reading frame III product and the coat protein is required for transmission of cauliflower mosaic virus by aphids.Journal of virology · 2001Article
- Mechanisms of arthropod transmission of plant and animal viruses.Microbiology and molecular biology reviews : MMBR · 1999Review
- The aphid transmission factor of cauliflower mosaic virus forms a stable complex with microtubules in both insect and plant cells.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
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7 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Cauliflower mosaic virus (CaMV) aphid transmission factor (ATF or P18) is presumed to interact with both virus particles and vector mouthparts, thereby mediating virus aphid transmission. We developed a protein-protein binding assay and our results clearly show that virus particles bind strongly and specifically to P18 whether P18 was obtained from plants, a baculovirus expression system, or the pGEX-3X Escherichia coli expression system. We overproduced, using the pGEX-3X expression system, various fragments of P18 and thereby demonstrated that the C-terminal 31 amino acid residues are responsible for the interaction. Using PCR-based mutagenesis, 2 amino acid residues essential for interaction were identified. Point substitutions (amino acids 157 from Ile to Asn or 159 from Gly to Ser) were sufficient to abolish the interaction, whereas another mutation (amino acid 158 from Ile to Ser) had no effect on P18 virus binding. We evaluated whether there was a correlation between the ability of P18 to interact with CaMV particles and its biological activity. Aphid transmission assays were carried out and we demonstrated that the loss of the virus binding capacity had a dramatic effect on the ability of P18 to mediate aphid transmission. Thus, our results suggest that binding between P18 and virus particles is likely to be one of the molecular mechanisms involved in CaMV aphid transmission.
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