ArticleThe Journal of cell biology1994
Human gamma-tubulin functions in fission yeast.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 64 citations in OpenAlex.
- The ring saga: looking back at the discovery of γ-tubulin and γ-tubulin ring complexes.Molecular biology of the cell · 2023Article
- γ-Tubulin in microtubule nucleation and beyond.Frontiers in cell and developmental biology · 2022Review
- Kinesin-14 and kinesin-5 antagonistically regulate microtubule nucleation by γ-TuRC in yeast and human cells.Nature communications · 2014Article
- Molecular insight into γ-γ tubulin lateral interactions within the γ-tubulin ring complex (γ-TuRC).Journal of computer-aided molecular design · 2014Article
- Fission yeast MOZART1/Mzt1 is an essential γ-tubulin complex component required for complex recruitment to the microtubule organizing center, but not its assembly.Molecular biology of the cell · 2013Article
- Kinesin-14 Pkl1 targets γ-tubulin for release from the γ-tubulin ring complex (γ-TuRC) .Cell cycle (Georgetown, Tex.) · 2013Article
- Distinct Dgrip84 isoforms correlate with distinct gamma-tubulins in Drosophila.Molecular biology of the cell · 2008Article
- FtsZ from divergent foreign bacteria can function for cell division in Escherichia coli.Journal of bacteriology · 2006Article
- Expression of Arabidopsis gamma-tubulin in fission yeast reveals conserved and novel functions of gamma-tubulin.Plant physiology · 2003Article
- Conditional mutations in gamma-tubulin reveal its involvement in chromosome segregation and cytokinesis.Molecular biology of the cell · 2001Article
- Identification of ribonucleotide reductase protein R1 as an activator of microtubule nucleation in Xenopus egg mitotic extracts.Molecular biology of the cell · 2000Article
- The fission yeast gamma-tubulin complex is required in G(1) phase and is a component of the spindle assembly checkpoint.The EMBO journal · 2000Article
- A mutation in gamma-tubulin alters microtubule dynamics and organization and is synthetically lethal with the kinesin-like protein pkl1p.Molecular biology of the cell · 2000Article
- A genetic analysis of interactions with Spc110p reveals distinct functions of Spc97p and Spc98p, components of the yeast gamma-tubulin complex.Molecular biology of the cell · 1998Article
- The mammalian gamma-tubulin complex contains homologues of the yeast spindle pole body components spc97p and spc98p.The Journal of cell biology · 1998Article
- Nucleation and capture of large cell surface-associated microtubule arrays that are not located near centrosomes in certain cochlear epithelial cells.Journal of anatomy · 1998Article
- Spc98p and Spc97p of the yeast gamma-tubulin complex mediate binding to the spindle pole body via their interaction with Spc110p.The EMBO journal · 1997Article
- Essential role for gamma-tubulin in the acentriolar female meiotic spindle of Drosophila.The EMBO journal · 1997Article
- The spindle pole body component Spc98p interacts with the gamma-tubulin-like Tub4p of Saccharomyces cerevisiae at the sites of microtubule attachment.The EMBO journal · 1996Article
- Analysis of Tub4p, a yeast gamma-tubulin-like protein: implications for microtubule-organizing center function.The Journal of cell biology · 1996Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
gamma-Tubulin is a phylogenetically conserved component of microtubule-organizing centers that is essential for viability and microtubule function. To examine the functional conservation of gamma-tubulin, we have tested the ability of human gamma-tubulin to function in the fission yeast Schizosaccharomyces pombe. We have found that expression of a human gamma-tubulin cDNA restores viability and a near-normal growth rate to cells of S. pombe lacking endogenous gamma-tubulin. Immunofluorescence microscopy showed that these cells contained normal mitotic spindles and interphase microtubule arrays, and that human gamma-tubulin, like S. pombe gamma-tubulin, localized to spindle pole bodies, the fungal microtubule-organizing centers. These results demonstrate that human gamma-tubulin functions in fission yeast, and they suggest that in spite of the great morphological differences between the microtubule-organizing centers of humans and fission yeasts, gamma-tubulin is likely to perform the same tasks in both. They suggest, moreover, that the proteins that interact with gamma-tubulin, including, most obviously, microtubule-organizing center proteins, must also be conserved. We have also found that a fivefold overexpression of S. pombe gamma-tubulin causes no reduction in growth rates or alteration of microtubule organization. We hypothesize that the excess gamma-tubulin is maintained in the cytoplasm in a form incapable of nucleating microtubule assembly. Finally, we have found that expression of human gamma-tubulin or overexpression of S. pombe gamma-tubulin causes no significant alteration of resistance to the antimicrotubule agents benomyl, thiabendazole and nocodazole.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.