ArticleThe Journal of cell biology1994
Microtubule dynamics in fish melanophores.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 76 citations in OpenAlex.
- Persistent growth of microtubules at low density.Molecular biology of the cell · 2021Article
- Photoactivatable fluorophores and techniques for biological imaging applications.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2012Review
- Switching of membrane organelles between cytoskeletal transport systems is determined by regulation of the microtubule-based transport.The Journal of cell biology · 2007Article
- Microtubule motor Ncd induces sliding of microtubules in vivo.Molecular biology of the cell · 2007Article
- Cytoskeletal dynamics during mammalian gametegenesis and fertilization: Implications for human reproduction.Reproductive medicine and biology · 2005Review
- Multiscale trend analysis of microtubule transport in melanophores.Biophysical journal · 2005Article
- Microtubule organization during rabbit fertilization by intracytoplasmic sperm injection with and without sperm centrosome.Reproductive medicine and biology · 2005Article
- Cytoplasmic dynein nucleates microtubules to organize them into radial arrays in vivo.Molecular biology of the cell · 2004Article
- Self-organization of a radial microtubule array by dynein-dependent nucleation of microtubules.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
- Region-specific microtubule transport in motile cells.The Journal of cell biology · 2000Article
- Mutations at phosphorylation sites of Xenopus microtubule-associated protein 4 affect its microtubule-binding ability and chromosome movement during mitosis.Molecular biology of the cell · 1999Article
- Centrosomal control of microtubule dynamics.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Transport and turnover of microtubules in frog neurons depend on the pattern of axonal growth.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Actomyosin-based retrograde flow of microtubules in the lamella of migrating epithelial cells influences microtubule dynamic instability and turnover and is associated with microtubule breakage and treadmilling.The Journal of cell biology · 1997Article
- Subcellular localization of myosin-V in the B16 melanoma cells, a wild-type cell line for the dilute gene.Molecular biology of the cell · 1997Article
- Molecular requirements for bi-directional movement of phagosomes along microtubules.The Journal of cell biology · 1997Article
- Microtubule dynamics at the G2/M transition: abrupt breakdown of cytoplasmic microtubules at nuclear envelope breakdown and implications for spindle morphogenesis.The Journal of cell biology · 1996Article
- Kinetochore microtubule dynamics and the metaphase-anaphase transition.The Journal of cell biology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
We have studied the dynamics of microtubules in black tetra (Gymnocorymbus ternetzi) melanophores to test the possible correlation of microtubule stability and intracellular particle transport. X-rhodamine-or caged fluorescein-conjugated tubulin were microinjected and visualized by fluorescence digital imaging using a cooled charge coupled device and videomicroscopy. Microtubule dynamics were evaluated by determining the time course of tubulin incorporation after pulse injection, by time lapse observation, and by quantitation of fluorescence redistribution after photobleaching and photoactivation. The time course experiments showed that the kinetics of incorporation of labeled tubulin into microtubules were similar for cells with aggregated or dispersed pigment with most microtubules becoming fully labeled within 15-20 min after injection. Quantitation by fluorescence redistribution after photobleaching and photoactivation confirmed that microtubule turnover was rapid in both states, t1/2 = 3.5 +/- 1.5 and 6.1 +/- 3.0 min for cells with aggregated and dispersed pigment, respectively. In addition, immunostaining with antibodies specific to posttranslationally modified alpha-tubulin, which is usually enriched in stable microtubules, showed that microtubules composed exclusively of detyrosinated tubulin were absent and microtubules containing acetylated tubulin were sparse. We conclude that the microtubules of melanophores are very dynamic, that their dynamic properties do not depend critically on the state of pigment distribution, and that their stabilization is not a prerequisite for intracellular transport.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.