ArticleThe Journal of cell biology1994
Ponticulin is an atypical membrane protein.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 53 citations in OpenAlex.
- RNABMC genomics · 2021Article
- Identification of Proteins Associated with Multilamellar Bodies Produced by Dictyostelium discoideum.PloS one · 2016Article
- Early Vertebrate Evolution of the Host Restriction Factor Tetherin.Journal of virology · 2015Article
- Counteraction of the multifunctional restriction factor tetherin.Frontiers in microbiology · 2014Review
- Article
- SrfB, a member of the Serum Response Factor family of transcription factors, regulates starvation response and early development in Dictyostelium.Developmental biology · 2008Article
- Self-assembly of actin scaffolds at ponticulin-containing supported phospholipid bilayers.Biophysical journal · 2006Article
- A transmembrane form of the prion protein contains an uncleaved signal peptide and is retained in the endoplasmic Reticulum.Molecular biology of the cell · 2001Article
- Characterization of the Wsc1 protein, a putative receptor in the stress response of Saccharomyces cerevisiae.Genetics · 1999Article
- Identification of a suppressor of the Dictyostelium profilin-minus phenotype as a CD36/LIMP-II homologue.The Journal of cell biology · 1999Article
- Interaptin, an actin-binding protein of the alpha-actinin superfamily in Dictyostelium discoideum, is developmentally and cAMP-regulated and associates with intracellular membrane compartments.The Journal of cell biology · 1998Article
- Identification of detergent-resistant plasma membrane microdomains in dictyostelium: enrichment of signal transduction proteins.Molecular biology of the cell · 1997Article
- Ordered yeast artificial chromosome clones representing the Dictyostelium discoideum genome.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- The Dictyostelium cytoskeleton.Experientia · 1995Review
- Ponticulin plays a role in the positional stabilization of pseudopods.The Journal of cell biology · 1995Article
- Targeting of Listeria monocytogenes ActA protein to the plasma membrane as a tool to dissect both actin-based cell morphogenesis and ActA function.The EMBO journal · 1995Article
- Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium.The Journal of cell biology · 1994Article
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Authors and funding
3 authors.
Funding
Abstract
We have cloned and sequenced ponticulin, a 17,000-dalton integral membrane glycoprotein that binds F-actin and nucleates actin assembly. A single copy gene encodes a developmentally regulated message that is high during growth and early development, but drops precipitously during cell streaming at approximately 8 h of development. The deduced amino acid sequence predicts a protein with a cleaved NH2-terminal signal sequence and a COOH-terminal glycosyl anchor. These predictions are supported by amino acid sequencing of mature ponticulin and metabolic labeling with glycosyl anchor components. Although no alpha-helical membrane-spanning domains are apparent, several hydrophobic and/or sided beta-strands, each long enough to traverse the membrane, are predicted. Although its location on the primary sequence is unclear, an intracellular domain is indicated by the existence of a discontinuous epitope that is accessible to antibody in plasma membranes and permeabilized cells, but not in intact cells. Such a cytoplasmically oriented domain also is required for the demonstrated role of ponticulin in binding actin to the plasma membrane in vivo and in vitro (Hitt, A. L., J. H. Hartwig, and E. J. Luna. 1994. Ponticulin is the major high affinity link between the plasma membrane and the cortical actin network in Dictyostelium. J. Cell Biol. 126:1433-1444). Thus, ponticulin apparently represents a new category of integral membrane proteins that consists of proteins with both a glycosyl anchor and membrane-spanning peptide domain(s).
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.