ArticleThe Journal of cell biology1994
Guanine nucleotide dissociation inhibitor is essential for Rab1 function in budding from the endoplasmic reticulum and transport through the Golgi stack.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
24 citing papers in PubMed, 84 citations in OpenAlex.
- Article
- Rab1 recruits WHAMM during membrane remodeling but limits actin nucleation.Molecular biology of the cell · 2016Article
- Article
- Article
- Mutant prion protein expression is associated with an alteration of the Rab GDP dissociation inhibitor alpha (GDI)/Rab11 pathway.Molecular & cellular proteomics : MCP · 2010Article
- Disruption of Rab11 activity in a knock-in mouse model of Huntington's disease.Neurobiology of disease · 2009Article
- Cholesterol controls lipid endocytosis through Rab11.Molecular biology of the cell · 2007Article
- Rabs and their effectors: achieving specificity in membrane traffic.Proceedings of the National Academy of Sciences of the United States of America · 2006Review
- The Hsp90 chaperone complex regulates GDI-dependent Rab recycling.Molecular biology of the cell · 2006Article
- The BAR domain proteins: molding membranes in fission, fusion, and phagy.Microbiology and molecular biology reviews : MMBR · 2006Review
- Molecular evolution of the Rab-escort-protein/guanine-nucleotide-dissociation-inhibitor superfamily.Molecular biology of the cell · 2003Article
- Rab11 is required for trans-golgi network-to-plasma membrane transport and a preferential target for GDP dissociation inhibitor.Molecular biology of the cell · 1998Article
- Initial docking of ER-derived vesicles requires Uso1p and Ypt1p but is independent of SNARE proteins.The EMBO journal · 1998Article
- Cargo selection by the COPII budding machinery during export from the ER.The Journal of cell biology · 1998Article
- The putative "switch 2" domain of the Ras-related GTPase, Rab1B, plays an essential role in the interaction with Rab escort protein.Molecular biology of the cell · 1998Article
- The mechanism of Golgi segregation during mitosis is cell type-specific.Proceedings of the National Academy of Sciences of the United States of America · 1997Article
- Homotypic lysosome fusion in macrophages: analysis using an in vitro assay.The Journal of cell biology · 1997Article
- Mitotic phosphorylation of rab4 prevents binding to a specific receptor on endosome membranes.The EMBO journal · 1997Article
- Mss4 does not function as an exchange factor for Rab in endoplasmic reticulum to Golgi transport.Molecular biology of the cell · 1997Article
- Two new Ypt GTPases are required for exit from the yeast trans-Golgi compartment.The Journal of cell biology · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
The small GTPase Rab1 is required for vesicular traffic from the ER to the cis-Golgi compartment, and for transport between the cis and medial compartments of the Golgi stack. In the present study, we examine the role of guanine nucleotide dissociation inhibitor (GDI) in regulating the function of Rab1 in the transport of vesicular stomatitis virus glycoprotein (VSV-G) in vitro. Incubation in the presence of excess GDI rapidly (t1/2 < 30 s) extracted Rab1 from membranes, inhibiting vesicle budding from the ER and sequential transport between the cis-, medial-, and trans-Golgi cisternae. These results demonstrate a direct role for GDI in the recycling of Rab proteins. Analysis of rat liver cytosol by gel filtration revealed that a major pool of Rab1 fractionates with a molecular mass of approximately 80 kD in the form of a GDI-Rab1 complex. When the GDI-Rab1 complex was depleted from cytosol by use of a Rab1-specific antibody, VSV-G failed to exit the ER. However, supplementation of depleted cytosol with a GDI-Rab1 complex prepared in vitro from recombinant forms of Rab1 and GDI efficiently restored export from the ER, and transport through the Golgi stack. These results provide evidence that a cytosolic GDI-Rab1 complex is required for the formation of non-clathrin-coated vesicles mediating transport through the secretory pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.