ArticleGenetics1994
Functional analyses of the Neurospora crassa MT a-1 mating type polypeptide.
Article in Genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 72 citations in OpenAlex.
- Inferring fungal cis-regulatory networks from genome sequences via unsupervised and interpretable representation learning.Genetics · 2026Article
- TheFrontiers in fungal biology · 2022Article
- The master regulator MAT1-1-1 of fungal mating binds to its targets via a conserved motif in the human pathogen Aspergillus fumigatus.G3 (Bethesda, Md.) · 2021Article
- Molecular Mechanisms Regulating Cell Fusion and Heterokaryon Formation in Filamentous Fungi.Microbiology spectrum · 2017Review
- Genetic Variation and Its Reflection on Posttranslational Modifications in Frequency Clock and Mating Type a-1 Proteins inBioMed research international · 2017Article
- Sex-determination system in the diploid yeast Zygosaccharomyces sapae.G3 (Bethesda, Md.) · 2014Article
- The MAT locus genes play different roles in sexual reproduction and pathogenesis in Fusarium graminearum.PloS one · 2013Article
- A network of HMG-box transcription factors regulates sexual cycle in the fungus Podospora anserina.PLoS genetics · 2013Article
- The role of pheromone receptors for communication and mating in Hypocrea jecorina (Trichoderma reesei).Fungal genetics and biology : FG & B · 2012Article
- Article
- Neurospora crassa mat A-2 and mat A-3 proteins weakly interact in the yeast two-hybrid system and affect yeast growth.Genetics and molecular biology · 2009Article
- The evolutionary trajectory of the mating-type (mat) genes in Neurospora relates to reproductive behavior of taxa.BMC evolutionary biology · 2008Article
- Microarray and real-time PCR analyses reveal mating type-dependent gene expression in a homothallic fungus.Molecular genetics and genomics : MGG · 2006Article
- Lessons from the genome sequence of Neurospora crassa: tracing the path from genomic blueprint to multicellular organism.Microbiology and molecular biology reviews : MMBR · 2004Review
- A G-protein beta subunit required for sexual and vegetative development and maintenance of normal G alpha protein levels in Neurospora crassa.Eukaryotic cell · 2002Article
- Molecular genetics of heterokaryon incompatibility in filamentous ascomycetes.Microbiology and molecular biology reviews : MMBR · 2000Review
- Molecular characterization of tol, a mediator of mating-type-associated vegetative incompatibility in Neurospora crassa.Genetics · 1999Article
- Molecular analysis of pcc1, a gene that leads to A-regulated sexual morphogenesis in Coprinus cinereus.Genetics · 1998Article
- Article
- Characterization of mat A-2, mat A-3 and deltamatA mating-type mutants of Neurospora crassa.Genetics · 1998Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Neurospora crassa mt a-1 gene, encoding the MT a-1 polypeptide, determines a mating type properties: sexual compatibility and vegetative incompatibility with A mating type. We characterized in vivo and in vitro functions of the MT a-1 polypeptide and specific mutant derivatives. MT a-1 polypeptide produced in Escherichia coli bound to specific DNA sequences whose core was 5'-CTTTG-3'. DNA binding was a function of the MT a-1 HMG box domain (a DNA binding motif found in high mobility group proteins and a diverse set of regulatory proteins). Mutation within the HMG box eliminated DNA binding in vitro and eliminated mating in vivo, but did not interfere with vegetative incompatibility function in vivo. Conversely, deletion of amino acids 216-220 of MT a-1 eliminated vegetative incompatibility, but did not affect mating or DNA binding. Deletion of the carboxyl terminal half of MT a-1 eliminated both mating and vegetative incompatibility in vivo, but not DNA binding in vitro. These results suggest that mating depends upon the ability of MT a-1 polypeptide to bind to, and presumably to regulate the activity of, specific DNA sequences. However, the separation of vegetative incompatibility from both mating and DNA binding indicates that vegetative incompatibility functions by a biochemically distinct mechanism.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.