ArticleThe Journal of clinical investigation1994
The endogenous vascular elastase that governs development and progression of monocrotaline-induced pulmonary hypertension in rats is a novel enzyme related to the serine proteinase adipsin.
Article in The Journal of clinical investigation, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 101 citations in OpenAlex.
- Role of complement factor D in cardiovascular and metabolic diseases.Frontiers in immunology · 2024Review
- Observational
- NAADP-induced intracellular calcium ion is mediated by the TPCs (two-pore channels) in hypoxia-induced pulmonary arterial hypertension.Journal of cellular and molecular medicine · 2021Article
- Hindrance of the Proteolytic Activity of Neutrophil-Derived Serine Proteases by Serine Protease Inhibitors as a Management of Cardiovascular Diseases and Chronic Inflammation.Frontiers in chemistry · 2021Review
- Inflammation in Pulmonary Arterial Hypertension.Advances in experimental medicine and biology · 2021Review
- Senescent dermal fibroblasts negatively influence fibroblast extracellular matrix-related gene expression partly via secretion of complement factor D.BioFactors (Oxford, England) · 2019Article
- The Role of Neutrophils and Neutrophil Elastase in Pulmonary Arterial Hypertension.Frontiers in medicine · 2018Review
- Brief Report: Association of Elevated Adipsin Levels With Pulmonary Arterial Hypertension in Systemic Sclerosis.Arthritis & rheumatology (Hoboken, N.J.) · 2017Article
- Serum Adipsin Levels throughout Normal Pregnancy and Preeclampsia.Scientific reports · 2016Article
- Challenges and Current Efforts in the Development of Biomarkers for Chronic Inflammatory and Remodeling Conditions of the Lungs.Biomarker insights · 2015Review
- Two sides of MGP null arterial disease: chondrogenic lesions dependent on transglutaminase 2 and elastin fragmentation associated with induction of adipsin.The Journal of biological chemistry · 2013Article
- Elevated fetal adipsin/acylation-stimulating protein (ASP) in obese pregnancy: novel placental secretion via Hofbauer cells.The Journal of clinical endocrinology and metabolism · 2013Article
- Endothelin-1, the unfolded protein response, and persistent inflammation: role of pulmonary artery smooth muscle cells.American journal of respiratory cell and molecular biology · 2012Article
- Pathogenesis of aortic dilatation in mucopolysaccharidosis VII mice may involve complement activation.Molecular genetics and metabolism · 2011Article
- Pulmonary vascular disease related to hemodynamic stress in the pulmonary circulation.Comprehensive Physiology · 2011Review
- Pathogenic mechanisms of pulmonary arterial hypertension.Journal of molecular and cellular cardiology · 2008Review
- One hundred years of research in the pathogenesis of pulmonary hypertension.American journal of respiratory cell and molecular biology · 2005Review
- Elastase and matrix metalloproteinase inhibitors induce regression, and tenascin-C antisense prevents progression, of vascular disease.The Journal of clinical investigation · 2000Article
- Targeted overexpression of elafin protects mice against cardiac dysfunction and mortality following viral myocarditis.The Journal of clinical investigation · 1999Article
- Expression of the elastolytic cathepsins S and K in human atheroma and regulation of their production in smooth muscle cells.The Journal of clinical investigation · 1998Article
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Authors and funding
9 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We showed previously a cause and effect relationship between increased activity of an endogenous vascular elastase (EVE) and experimentally induced pulmonary hypertension in rats. We now report the isolation and characterization of EVE. Degenerate oligonucleotides synthesized to homologous sequences in serine elastases were used in a PCR with rat pulmonary artery (PA) cDNA. The PCR product hybridized to a 1.2-kb mRNA and the intensity of hybridization was threefold increased in RNA from rat hypertensive PA at a timepoint when EVE activity was increased. The PCR product was used to screen a cDNA library and sequences obtained encoded rat adipsin. We then used immunoaffinity to purify EVE. An antibody to the elastin-binding protein was used to remove this competitor of elastase from the PA extract and the elastolytic activity increased 100-fold. The enzyme was purified using an antibody that recognizes NH2-terminal sequences of serine proteinases and the eluate was further purified using an antibody raised against recombinant adipsin. A single band at 20 kD immunoreactive with the adipsin antibody was resolved as an active enzyme on an elastin substrate gel. Immunogold labeling with an antibody to an adipsin peptide sequence localized EVE to PA smooth muscle cells. This is the first isolation of EVE; it appears to be a novel enzyme related to the serine proteinase adipsin originally found in adipose tissue.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.