ArticleJournal of bacteriology1994
PrlA and PrlG suppressors reduce the requirement for signal sequence recognition.
Article in Journal of bacteriology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 33 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
33 citing papers in PubMed, 76 citations in OpenAlex.
- SecY-mediated quality control prevents the translocation of non-gated porins.Scientific reports · 2020Article
- SecA-Mediated Protein Translocation through the SecYEG Channel.Microbiology spectrum · 2019Review
- Genetic Analysis of Protein Translocation.The protein journal · 2019Review
- The Sec System: Protein Export inEcoSal Plus · 2017Review
- Conformational Changes That Coordinate the Activity of BamA and BamD Allowing β-Barrel Assembly.Journal of bacteriology · 2017Article
- Article
- Selective transport by SecA2: an expanding family of customized motor proteins.Biochimica et biophysica acta · 2014Review
- The bacterial Sec-translocase: structure and mechanism.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2012Review
- Tail-anchored membrane protein insertion into the endoplasmic reticulum.Nature reviews. Molecular cell biology · 2011Review
- Transport of preproteins by the accessory Sec system requires a specific domain adjacent to the signal peptide.Journal of bacteriology · 2010Article
- Article
- Folding and trimerization of signal sequence-less mature TolC in the cytoplasm of Escherichia coli.Microbiology (Reading, England) · 2009Article
- Interactions that drive Sec-dependent bacterial protein transport.Biochemistry · 2007Review
- SPdb--a signal peptide database.BMC bioinformatics · 2005Article
- Investigating the SecY plug movement at the SecYEG translocation channel.The EMBO journal · 2005Article
- Modeling the effects of prl mutations on the Escherichia coli SecY complex.Journal of bacteriology · 2005Article
- A little help from my friends: quality control of presecretory proteins in bacteria.Journal of bacteriology · 2004Review
- Ring-like pore structures of SecA: implication for bacterial protein-conducting channels.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Recognition of secretory proteins in Escherichia coli requires signals in addition to the signal sequence and slow folding.BMC microbiology · 2002Article
- Critical regions of secM that control its translation and secretion and promote secretion-specific secA regulation.Journal of bacteriology · 2002Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Selection for suppressors of defects in the signal sequence of secretory proteins has led most commonly to identification of prlA alleles and less often to identification of prlG alleles. These genes, secY/prlA and secE/prlG, encode integral membrane components of the protein translocation system of Escherichia coli. We demonstrate that an outer membrane protein, LamB, that lacks a signal sequence can be exported with reasonable efficiency in both prlA and prlG suppressor strains. Although the signal sequence is not absolutely required for export of LamB, the level of export in the absence of prl suppressor alleles is exceedingly low. Such strains are phenotypically LamB-, and functional LamB can be detected only by using sensitive infectious-center assays. Suppression of the LamB signal sequence deletion is dependent on normal components of the export pathway, indicating that suppression is not occurring through a bypass mechanism. Our results indicate that the majority of the known prlA suppressors function by an identical mechanism and, further, that the prlG suppressors work in a similar fashion. We propose that both PrlA and PrlG suppressors lack a proofreading activity that normally rejects defective precursors from the export pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.