ArticleNucleic acids research1994
Directly labeled DNA probes using fluorescent nucleotides with different length linkers.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 136 citations in OpenAlex.
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- Comparison of pre-labelled primers and nucleotides as DNA labelling method for lateral flow detection of Legionella pneumophila amplicons.Scientific reports · 2024Article
- Multilabel hybridization probes for sequence-specific detection of sepsis-related drug resistance genes in plasmids.Talanta open · 2021Article
- DNA bridges: A novel platform for single-molecule sequencing and other DNA-protein interaction applications.PloS one · 2021Article
- FISH Going Meso-Scale: A Microscopic Search for Chromatin Domains.Frontiers in cell and developmental biology · 2021Review
- Ultrasensitive THz biosensor for PCR-free cDNA detection based on frequency selective surfaces.Biomedical optics express · 2020Article
- Synthesis of Fluorescence Turn-On DNA Hybridization Probe Using theCurrent protocols in nucleic acid chemistry · 2018Article
- Antibody-nucleotide conjugate as a substrate for DNA polymerases.Chemical science · 2018Article
- dUTPs conjugated with zwitterionic Cy3 or Cy5 fluorophore analogues are effective substrates for DNA amplification and labelling by Taq polymerase.Nucleic acids research · 2018Article
- Comparative Study of Novel Fluorescent Cyanine Nucleotides: Hybridization Analysis of Labeled PCR Products Using a Biochip.Journal of fluorescence · 2017Article
- Efficient enzymatic synthesis and dual-colour fluorescent labelling of DNA probes using long chain azido-dUTP and BCN dyes.Nucleic acids research · 2016Article
- The Toolbox for Modified Aptamers.Molecular biotechnology · 2016Review
- Functionalized tricyclic cytosine analogues provide nucleoside fluorophores with improved photophysical properties and a range of solvent sensitivities.Chemistry (Weinheim an der Bergstrasse, Germany) · 2014Article
- Artificial specific binders directly recovered from chemically modified nucleic acid libraries.Journal of nucleic acids · 2012Article
- Synthesis of the 5'-Se-thymidine phosphoramidite and convenient labeling of DNA oligonucleotide.Organic letters · 2011Article
- An integrated system for DNA sequencing by synthesis using novel nucleotide analogues.Accounts of chemical research · 2010Article
- CyDNA: synthesis and replication of highly Cy-dye substituted DNA by an evolved polymerase.Journal of the American Chemical Society · 2010Article
- Photoinitiator nucleotide for quantifying nucleic Acid hybridization.Biomacromolecules · 2010Article
- Synthesis of a new NIR fluorescent Nd complex labeling agent.Journal of fluorescence · 2010Article
- Long, processive enzymatic DNA synthesis using 100% dye-labeled terminal phosphate-linked nucleotides.Nucleosides, nucleotides & nucleic acids · 2008Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Directly labeled fluorescent DNA probes have been made by nick translation and PCR using dUTP attached to the fluorescent label, Cy3, with different length linkers. With preparation of probes by PCR we find that linker length affects the efficiency of incorporation of Cy3-dUTP, the yield of labeled probe, and the signal intensity of labeled probes hybridized to chromosome target sequences. For nick translation and PCR, both the level of incorporation and the hybridization fluorescence signal increased in parallel when the length of the linker arm is increased. Under optimal conditions, PCR yielded more densely labeled probes, however, the yield of PCR labeled probe decreased with greater linear density of labeling. By using a Cy3-modified dUTP with the longest linker under optimal conditions it was possible to label up to 28% of the possible substitution sites on the target DNA with reasonable yield by PCR and 18% by nick translation. A mechanism involving steric interactions between the polymerase, cyanine-labeled sites on template and extending chains and the modified dUTP substrate is proposed to explain the inverse correlation between the labeling efficiency and the yield of DNA probe synthesis by PCR.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.