Effects of 2-chloropropionate on venous plasma lactate concentration and anaerobic power during periods of incremental intensive exercise in humans.

We investigated the effects of a stimulation of pyruvate dehydrogenase (PDH) activity induced by 2-chloropropionate (2-CP) on venous plasma lactate concentration and peak anaerobic power (W an, peak) during periods (6 s) of incremental intense exercise, i.e. a force-velocity (F-v) test known to induce a marked accumulation of lactate in the blood. The F-v test was performed twice by six subjects according to a double-blind randomized crossover protocol: once with placebo and once with 2-CP (43 mg.kg-1 body mass). Blood samples were taken at ingestion of the drug, at 10, 20, and 40 min into the pretest period, at the end of each period of intense exercise, at the end of each 5-min recovery period, and after completion of the F-v test at 5, 10, 15, and 30 min. During the F-v test, venous plasma lactate concentrations with both placebo and 2-CP increased significantly when measured at the end of each period of intense exercise (F = 33.5, P < 0.001), and each 5-min recovery period (F = 24.6, P < 0.001). Venous plasma lactate concentrations were significantly lower with 2-CP at the end of each recovery period (P < 0.01), especially for high braking forces, i.e. 8 kg (P < 0.05), 9 kg (P < 0.02), and maximal braking force (P < 0.05). After completion of the F-v test, venous plasma lactate concentrations were also significantly lower with 2-CP (P < 0.001). The percentage of lactate decrease between 5- and 30-min recovery was significantly higher with 2-CP than with the placebo [59 (SEM 4)% vs 44.6 (SEM 5.5)%, P < 0.05]. Furthermore, W an, peak was significantly higher with 2-CP than with the placebo [1016 (SEM 60) W vs 957 (SEM 55) W, P < 0.05]. In conclusion, PDH activation by 2-CP attenuated the increase in venous plasma lactate concentration during the F-v test. Ingestion of 2-CP led to an increased W an, peak.