ArticleThe EMBO journal1994
Evidence that HIV-1 Rev directly promotes the nuclear export of unspliced RNA.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 114 papers.
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Who cites it
114 citing papers in PubMed, 244 citations in OpenAlex.
- High-titer modular retroviral vectors enabled by an antisense cassette design preventing dsRNA formation during virus production.Molecular therapy. Advances · 2026Article
- Review
- Review
- Virus Infection and mRNA Nuclear Export.International journal of molecular sciences · 2023Review
- Sending the message: specialized RNA export mechanisms in trypanosomes.Trends in parasitology · 2022Review
- Human Retrovirus Genomic RNA Packaging.Viruses · 2022Review
- The nuclear transcription factor, TAF7, is a cytoplasmic regulator of protein synthesis.Science advances · 2021Article
- HIV Rev-isited.Open biology · 2020Review
- Barriers for HIV Cure: The Latent Reservoir.AIDS research and human retroviruses · 2018Review
- Eliminating HIV-1 Packaging Sequences from Lentiviral Vector Proviruses Enhances Safety and Expedites Gene Transfer for Gene Therapy.Molecular therapy : the journal of the American Society of Gene Therapy · 2017Article
- HIV Cell-to-Cell Spread Results in Earlier Onset of Viral Gene Expression by Multiple Infections per Cell.PLoS pathogens · 2016Article
- HIV-1 endocytosis in astrocytes: a kiss of death or survival of the fittest?Neuroscience research · 2014Review
- Allosteric control of the exportin CRM1 unraveled by crystal structure analysis.The FEBS journal · 2014Review
- Article
- HIV-1 Rev protein specifies the viral RNA export pathway by suppressing TAP/NXF1 recruitment.Nucleic acids research · 2014Article
- Endocytosis-mediated HIV-1 entry and its significance in the elusive behavior of the virus in astrocytes.Virology · 2014Article
- Unperturbed posttranscriptional regulatory Rev protein function and HIV-1 replication in astrocytes.PloS one · 2014Article
- Nuclear trafficking of retroviral RNAs and Gag proteins during late steps of replication.Viruses · 2013Review
- NESdb: a database of NES-containing CRM1 cargoes.Molecular biology of the cell · 2012Article
- Formation of trans-activation competent HIV-1 Rev:RRE complexes requires the recruitment of multiple protein activation domains.PloS one · 2012Article
54 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Rev trans-activator of human immunodeficiency virus type 1 (HIV-1) is a protein that regulates the simultaneous appearance in the cytoplasm of both spliced and unspliced forms of viral mRNAs from the same viral transcripts by way of recognition of a target sequence termed the Rev-responsive element (RRE). Whether Rev acts directly on RNA export or by inhibition of splicing, or both, is still a matter of debate. We have addressed this issue in Xenopus laevis oocytes by microinjecting RNA molecules containing RRE along with purified recombinant Rev protein into the oocyte nuclei. Adenovirus pre-mRNA containing an RRE in the intron was spliced equally well in the absence and presence of Rev protein. Only in the presence of Rev was non-spliced pre-mRNA exported from the nucleus; more surprisingly, the excised intron lariat (containing RRE) was also exported. Furthermore, an RRE-containing mRNA molecule that lacked intron sequences was also efficiently exported from the nucleus in a Rev-dependent manner. Therefore our results demonstrate that Rev can act directly at the level of nuclear export, independent of any inhibitory effect that it may exert on the splicing of pre-mRNA. Finally, our finding that the Rev mutant M10, shown previously to be inactive in human lymphoid cells, was also unable to export RRE-containing RNA molecules from oocyte nuclei suggests that one or more cellular factors, evolutionarily conserved between humans and Xenopus, interact with Rev in both cell systems to promote nuclear RNA export.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.