ArticleThe EMBO journal1994
CCAAT box binding protein NF-Y facilitates in vivo recruitment of upstream DNA binding transcription factors.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
48 citing papers in PubMed, 138 citations in OpenAlex.
- Article
- Genome-wide CIITA-binding profile identifies sequence preferences that dictate function versus recruitment.Nucleic acids research · 2015Article
- Arabidopsis DPB3-1, a DREB2A interactor, specifically enhances heat stress-induced gene expression by forming a heat stress-specific transcriptional complex with NF-Y subunits.The Plant cell · 2014Article
- Identifying and mapping cell-type-specific chromatin programming of gene expression.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Bile acids repress E-cadherin through the induction of Snail and increase cancer invasiveness in human hepatobiliary carcinoma.Cancer science · 2008Article
- Dynamics of global transcriptome in bovine matured oocytes and preimplantation embryos.Proceedings of the National Academy of Sciences of the United States of America · 2006Article
- Transcriptional modulation of the pre-implantation embryo-specific Rnf35 gene by the Y-box protein NF-Y/CBF.The Biochemical journal · 2005Article
- Inhibition of MHC II gene transcription by nitric oxide and antioxidants.Current pharmaceutical design · 2004Review
- Expression of nonclassical MHC class Ib genes: comparison of regulatory elements.Immunologic research · 2003Review
- Functional interaction of nuclear factor y and sp1 is required for activation of the epstein-barr virus C promoter.Journal of virology · 2003Article
- LEAFY COTYLEDON1-LIKE defines a class of regulators essential for embryo development.The Plant cell · 2003Article
- DEK binding to class II MHC Y-box sequences is gene- and allele-specific.Arthritis research & therapy · 2003Article
- Novel mechanisms of class II major histocompatibility complex gene regulation.Immunologic research · 2003Review
- Transcriptional regulation of porcine endogenous retroviruses released from porcine and infected human cells by heterotrimeric protein complex NF-Y and impact of immunosuppressive drugs.Journal of virology · 2002Article
- Characterization of the human ephrin-A4 promoter.The Biochemical journal · 2002Article
- Impaired regulation of HLA-DR expression in human immunodeficiency virus-infected monocytes.Clinical and diagnostic laboratory immunology · 2002Review
- Murine NFX.1: isolation and characterization of its messenger RNA, mapping of its chromosomal location and assessment of its developmental expression.Immunology · 2002Article
- Promoter-specific functions of CIITA and the MHC class II enhanceosome in transcriptional activation.The EMBO journal · 2002Article
- Function and regulation of class II transactivator in the immune system.Immunologic research · 2002Review
- NF-Y regulates LIF-induced transcription of the signaling adaptor SKAP55R in myeloid cells.Leukemia · 2001Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
NF-Y binds a CCAAT motif found in many eukaryotic polymerase II-dependent promoters. In the HLA-DRA promoter it has been demonstrated that stereo-specific alignment between this motif and the upstream elements X1 and X2 is required for activation. To study the underlying mechanism for this requirement, a panel of transfected cell lines that maintained integrated, wild-type and mutant promoters were analyzed by in vivo genomic footprinting. Cell lines harboring a mutated CCAAT element exhibited a loss of interactions at the CCAAT site, as expected, and no transcriptional activity. Most importantly, mutation of the CCAAT sequence nearly abolished in vivo binding at the X1 and X2 sites, while mutations of X1 and X2 had little effect on CCAAT box binding. However, X1 and X2 binding was interdependent. In vitro, X1 binding activities are known to be stabilized by NF-Y binding. Interaction between NF-Y and X box binding proteins was demonstrated by reciprocal co-immunoprecipitation in the absence of DNA and co-affinity purification in the presence of DNA. Collectively, these studies indicate that occupancy of the CCAAT element represents an early event affecting other protein-DNA interactions and suggest that NF-Y stabilizes and interacts with X box factors to mediate this function. These findings may represent a common theme among promoters containing a CCAAT element.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.