ArticleBritish journal of pharmacology1994
Blockade by 4,4'-diisothiocyanatostilbene-2,2'-disulphonate (DIDS) of P2X-purinoceptors in rat vas deferens.
Article in British journal of pharmacology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 50 citations in OpenAlex.
- Isothiocyanatostilbenes as novel c-Met inhibitors.Oncotarget · 2015Article
- Effect of type 1 diabetes on the production and vasoactivity of hydrogen sulfide in rat middle cerebral arteries.Physiological reports · 2013Article
- PI3K and PKC contribute to membrane depolarization mediated by alpha2-adrenoceptors in the canine isolated mesenteric vein.BMC physiology · 2005Article
- Purine and pyrimidine (P2) receptors as drug targets.Journal of medicinal chemistry · 2002Review
- Extracellular ATP activates calcium signaling, ion, and fluid transport in retinal pigment epithelium.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1997Article
- P2-purinoceptor antagonists: II. Blockade of P2-purinoceptor subtypes and ecto-nucleotidases by compounds related to Evans blue and trypan blue.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
- P2-purinoceptor antagonists: I. Blockade of P2-purinoceptor subtypes and ecto-nucleotidases by small aromatic isothiocyanato-sulphonates.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
- A comparison of the binding characteristics of recombinant P2X1 and P2X2 purinoceptors.British journal of pharmacology · 1996Article
- Acute paw oedema formation induced by ATP: re-evaluation of the mechanisms involved.Inflammation research : official journal of the European Histamine Research Society ... [et al.] · 1996Article
- Failure of tyramine to release neuronal ATP as a cotransmitter of noradrenaline in the guinea-pig vas deferens.Naunyn-Schmiedeberg's archives of pharmacology · 1996Article
- New insights on P2X purinoceptors.Naunyn-Schmiedeberg's archives of pharmacology · 1995Review
- Reactive red 2: a P2y-selective purinoceptor antagonist and an inhibitor of ecto-nucleotidase.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- DIDS increases K+ secretion through an IsK channel in apical membrane of vestibular dark cell epithelium of gerbil.The Journal of membrane biology · 1995Article
- Inhibitory action of PPADS on relaxant responses to adenine nucleotides or electrical field stimulation in guinea-pig taenia coli and rat duodenum.British journal of pharmacology · 1995Article
- Discrimination between UTP- and P2-purinoceptor-mediated depolarization of rat superior cervical ganglia by 4,4'-diisothiocyanatostilbene-2,2'- disulphonate (DIDS) and uniblue A.British journal of pharmacology · 1995Article
- Two relaxation-mediating P2-purinoceptors in guinea-pig taenia caeci.Naunyn-Schmiedeberg's archives of pharmacology · 1995Article
- Release of ATP in rat vas deferens: origin and role of calcium.Naunyn-Schmiedeberg's archives of pharmacology · 1994Article
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Authors and funding
2 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
1. The possibility of an antagonist effect of 4,4'-diisothiocyanatostilbene-2,2'-disulphonate (DIDS) at P2X-purinoceptors was studied in rat vas deferens. 2. DIDS reduced contractions elicited by alpha,beta-methylene ATP 3 microM, IC50 1.6 microM, but did not change contractions elicited by K+ 35 mM. DIDS 3.2 microM slightly shifted the concentration-response curve of alpha,beta-methylene ATP to the right and reduced the maximum. DIDS 10 microM markedly decreased and DIDS 32 microM abolished contractions over the entire range of the alpha, beta-methylene ATP concentration-response curve. DIDS 32 microM also abolished contractions elicited by ATP but did not change contractions elicited by noradrenaline. The antagonist effect of DIDS was only slowly reversible. 3. The presence of either suramin 320 microM or alpha,beta-methylene ATP 10 microM during the exposure to DIDS protected the tissue from the long-lasting blocking effect of DIDS. 4. 4,4'-Diisothiocyanatodihydrostilbene-2,2'-disulphonate (H2DIDS) was equipotent with DIDS whereas several analogues in which one or both of the isothiocyanate residues were replaced were less effective or without effect against alpha,beta-methylene ATP. 5. DIDS attenuated the purinergic component of neurogenic contractions elicited by electrical field stimulation, IC50 3.9 microM, but did not change the adrenergic component. 6. It is concluded that DIDS causes a selective, long-lasting, non-equilibrium blockade of P2X-purinoceptors in rat vas deferens. Due to this effect it also selectively blocks the purinergic component of neurogenic contractions.
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