ArticleApplied and environmental microbiology1994
Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum.
Article in Applied and environmental microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 66 citations in OpenAlex.
- Coniferyl aldehyde from the phenylpropanoid pathway targets pyruvate kinase in Dactylobotrys graminicola to confer sheath rot resistance in hulless barley.Journal of integrative plant biology · 2026Article
- Pyruvate kinase 2 from Synechocystis sp. PCC 6803 increased substrate affinity via glucose-6-phosphate and ribose-5-phosphate for phosphoenolpyruvate consumption.Plant molecular biology · 2024Article
- Microbial synthesis of the plant natural product precursor p-coumaric acid with Corynebacterium glutamicum.Microbial cell factories · 2023Article
- The KInternational journal of molecular sciences · 2022Article
- Metabolic engineering of carbohydrate metabolism systems in Corynebacterium glutamicum for improving the efficiency of L-lysine production from mixed sugar.Microbial cell factories · 2020Article
- Continuous Adaptive Evolution of a Fast-GrowingFrontiers in microbiology · 2019Article
- The FFrontiers in microbiology · 2018Article
- Article
- A novel pyruvate kinase and its application in lactic acid production under oxygen deprivation in Corynebacterium glutamicum.BMC biotechnology · 2016Article
- Turning the respiratory flexibility of Mycobacterium tuberculosis against itself.Nature communications · 2016Article
- Effect of pyruvate kinase gene deletion on the physiology ofMetabolic engineering communications · 2015Article
- New insights on the mechanism of the K(+-) independent activity of crenarchaeota pyruvate kinases.PloS one · 2015Article
- Role of Corynebacterium glutamicum sprA encoding a serine protease in glxR-mediated global gene regulation.PloS one · 2014Article
- Genome-scale metabolic network guided engineering of Streptomyces tsukubaensis for FK506 production improvement.Microbial cell factories · 2013Article
- The global repressor SugR controls expression of genes of glycolysis and of the L-lactate dehydrogenase LdhA in Corynebacterium glutamicum.Journal of bacteriology · 2008Article
- NCgl2620 encodes a class II polyphosphate kinase in Corynebacterium glutamicum.Applied and environmental microbiology · 2007Article
- Proteins from extremophiles as stable tools for advanced biotechnological applications of high social interest.Journal of the Royal Society, Interface · 2007Review
- Pyruvate:quinone oxidoreductase from Corynebacterium glutamicum: purification and biochemical characterization.Journal of bacteriology · 2005Article
- Identification and characterization of glxR, a gene involved in regulation of glyoxylate bypass in Corynebacterium glutamicum.Journal of bacteriology · 2004Article
- In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome.Journal of bacteriology · 2004Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Pyruvate kinase activity is an important element in the flux control of the intermediate metabolism. The purified enzyme from Corynebacterium glutamicum demonstrated a marked sigmoidal dependence of the initial rate on the phosphoenolpyruvate concentration. In the presence of the negative allosteric effector ATP, the phosphoenolpyruvate concentration at the half-maximum rate (S0.5) increased from 1.2 to 2.8 mM, and cooperation, as expressed by the Hill coefficient, increased from 2.0 to 3.2. AMP promoted opposite effects: the S0.5 was decreased to 0.4 mM, and the enzyme exhibited almost no cooperation. The maximum reaction rate was 702 U/mg, which corresponded to an apparent kcat of 2,540 s-1. The enzyme was not influenced by fructose-1,6-diphosphate and used Mn2+ or Co2+ as cations. Sequence determination of the C. glutamicum pyk gene revealed an open reading frame coding for a polypeptide of 475 amino acids. From this information and the molecular mass of the native protein, it follows that the pyruvate kinase is a tetramer of 236 kDa. Comparison of the deduced polypeptide sequence with the sequences of other bacterial pyruvate kinases showed 39 to 44% homology, with some regions being very strongly conserved.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.