ArticleGenetics1994
Unrepaired heteroduplex DNA in Saccharomyces cerevisiae is decreased in RAD1 RAD52-independent recombination.
Article in Genetics, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 62 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
62 citing papers in PubMed, 81 citations in OpenAlex.
- Nonhomologous tails direct heteroduplex rejection and mismatch correction during single-strand annealing in Saccharomyces cerevisiae.PLoS genetics · 2024Article
- Deficiency in homologous recombination is associated with changes in cell cycling and morphology in Saccharomyces cerevisiae.Experimental cell research · 2023Article
- Single-strand template repair: key insights to increase the efficiency of gene editing.Current genetics · 2021Review
- A Rad51-independent pathway promotes single-strand template repair in gene editing.PLoS genetics · 2020Article
- Partner Choice in Spontaneous Mitotic Recombination in Wild Type and Homologous Recombination Mutants ofG3 (Bethesda, Md.) · 2019Article
- Constitutively active Artemis nuclease recognizes structures containing single-stranded DNA configurations.DNA repair · 2019Article
- An Assay to Study Intra-Chromosomal Deletions in Yeast.Methods and protocols · 2019Article
- Increased chromosomal mobility after DNA damage is controlled by interactions between the recombination machinery and the checkpoint.Genes & development · 2018Article
- Article
- The homologous recombination protein RAD51D protects the genome from large deletions.Nucleic acids research · 2017Article
- Homology Requirements and Competition between Gene Conversion and Break-Induced Replication during Double-Strand Break Repair.Molecular cell · 2017Article
- Review
- Recombination and annealing pathways compete for substrates in making rrn duplications in Salmonella enterica.Genetics · 2014Article
- To nick or not to nick: comparison of I-SceI single- and double-strand break-induced recombination in yeast and human cells.PloS one · 2014Article
- Roles of XRCC2, RAD51B and RAD51D in RAD51-independent SSA recombination.PLoS genetics · 2013Article
- The Shu complex interacts with Rad51 through the Rad51 paralogues Rad55-Rad57 to mediate error-free recombination.Nucleic acids research · 2013Article
- Rad59 regulates association of Rad52 with DNA double-strand breaks.MicrobiologyOpen · 2012Article
- Article
- Article
- Processing of joint molecule intermediates by structure-selective endonucleases during homologous recombination in eukaryotes.Chromosoma · 2011Review
2 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
A direct repeat recombination assay between SUP4 heteroalleles detects unrepaired heteroduplex DNA (hDNA) as sectored colonies. The frequency of unrepaired heteroduplex is dependent on the mismatch and is highest in a construct that generates C:C or G:G mispairs and lowest in one that generates T:G or C:A mispairs. In addition, unrepaired hDNA increases for all mismatches tested in pms1 mismatch repair-deficient strains. These results support the notion that hDNA is formed across the SUP4 repeats during the recombination event and is then subject to mismatch repair. The effects of various repair and recombination defective mutations on this assay were examined. Unrepaired heteroduplex increases significantly only in rad52 mutant strains. In addition, direct repeat recombination is reduced 2-fold in rad52 mutant strains, while in rad51, rad54, rad55 and rad57 mutants direct repeat recombination is increased 3-4-fold. Mutations in the excision repair gene, RAD1, do not affect the frequency of direct repeat recombination. However, the level of unrepaired heteroduplex is slightly decreased in rad1 mutant strains. Similar to previous studies, rad1 rad52 double mutants show a synergistic reduction in direct repeat recombination (35-fold). Interestingly, unrepaired heteroduplex is reduced 4-fold in the double mutants. Experiments with shortened repeats suggest that the reduction in unrepaired heteroduplex is due to decreased hDNA tract length in the double mutant strain.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.