ArticleThe EMBO journal1994
Requirement for a zinc motif for template recognition by the bacteriophage T7 primase.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 70 citations in OpenAlex.
- Elucidation of DNA Primase as a Drug Target in Leishmania donovani: Structure-Guided Inhibitor Identification and Biochemical Validation.Chemical biology & drug design · 2026Article
- 3'dNTP Binding Is Modulated during Primer Synthesis and Translesion by Human PrimPol.International journal of molecular sciences · 2023Article
- The three-component helicase/primase complex of herpes simplex virus-1.Open biology · 2021Review
- Implications of Membrane Binding by the Fe-S Cluster-Containing N-Terminal Domain in theFrontiers in genetics · 2021Article
- DnaG Primase-A Target for the Development of Novel Antibacterial Agents.Antibiotics (Basel, Switzerland) · 2018Review
- Optimal numbers of residues in linkers of DNA polymerase I, T7 primase and DNA polymerase IV.Scientific reports · 2016Article
- Molecular dissection of the domain architecture and catalytic activities of human PrimPol.Nucleic acids research · 2014Article
- Zinc-binding domain of the bacteriophage T7 DNA primase modulates binding to the DNA template.The Journal of biological chemistry · 2012Article
- Binding mechanism of metal⋅NTP substrates and stringent-response alarmones to bacterial DnaG-type primases.Structure (London, England : 1993) · 2012Article
- The roles of tryptophans in primer synthesis by the DNA primase of bacteriophage T7.The Journal of biological chemistry · 2012Article
- Herpes simplex virus type 1 helicase-primase: DNA binding and consequent protein oligomerization and primase activation.Journal of virology · 2011Article
- Communication between the zinc and nickel sites in dimeric HypA: metal recognition and pH sensing.Journal of the American Chemical Society · 2010Article
- Mechanism of sequence-specific template binding by the DNA primase of bacteriophage T7.Nucleic acids research · 2010Article
- Direct role for the RNA polymerase domain of T7 primase in primer delivery.Proceedings of the National Academy of Sciences of the United States of America · 2010Article
- Mechanism and evolution of DNA primases.Biochimica et biophysica acta · 2010Review
- Functional importance of the conserved N-terminal domain of the mitochondrial replicative DNA helicase.Biochimica et biophysica acta · 2009Article
- DNA recognition by the DNA primase of bacteriophage T7: a structure-function study of the zinc-binding domain.Biochemistry · 2009Article
- A mutation in the human herpes simplex virus type 1 UL52 zinc finger motif results in defective primase activity but can recruit viral polymerase and support viral replication efficiently.Journal of virology · 2007Article
- Simultaneous amplification and screening of whole plasmids using the T7 bacteriophage replisome.Nucleic acids research · 2006Article
- Regulation of bacterial priming and daughter strand synthesis through helicase-primase interactions.Nucleic acids research · 2006Review
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Gene 4 of bacteriophage T7 encodes two proteins, a 63 kDa and a colinear 56 kDa protein. The coding sequence of the 56 kDa protein begins at the residues encoding an internal methionine located 64 amino acids from the N-terminus of the 63 kDa protein. The 56 kDa gene 4 protein is a helicase and the 63 kDa gene 4 protein is a helicase and a primase. The unique 7 kDa N-terminus of the 63 kDa gene 4 protein is essential for primer synthesis and contains sequences with homology to a Cys4 metal binding motif, Cys-X2-Cys-X17-Cys-X2-Cys. The zinc content of the 63 kDa gene 4 protein is 1.1 g-atom/mol protein, while the zinc content of the 56 kDa gene 4 protein is < 0.01, as determined by atomic absorption spectrometry. A bacteriophage deleted for gene 4, T7 delta 4-1, is incapable of growing on Escherichia coli strains that contain plasmids expressing gene 4 proteins with single amino acid substitutions of Ser at each of the four conserved Cys residues (efficiency of plating, 10(-7)). Primase containing a substitution of the third Cys for Ser has been overexpressed in E. coli and purified to homogeneity. This mutant primase cannot catalyze template-directed synthesis of oligoribonucleotides although it is able to catalyze the synthesis of random diribonucleotides in a template-independent fashion. The mutant primase has reduced helicase activity although it catalyzes single-stranded DNA-dependent hydrolysis of dTTP at rates comparable with wild type primase. The zinc content of the mutant primase is 0.5 g-atom/mol protein.
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