ArticleNucleic acids research1994
An adenovirus E1A transcriptional repressor domain functions as an activator when tethered to a promoter.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 37 citations in OpenAlex.
- Genomic Evolution and Recombination Dynamics of Human Adenovirus D Species: Insights from Comprehensive Bioinformatic Analysis.Journal of microbiology (Seoul, Korea) · 2024Review
- Article
- Mimicry of Cellular A Kinase-Anchoring Proteins Is a Conserved and Critical Function of E1A across Various Human Adenovirus Species.Journal of virology · 2018Article
- Viral retasking of hBre1/RNF20 to recruit hPaf1 for transcriptional activation.PLoS pathogens · 2013Article
- Cellular GCN5 is a novel regulator of human adenovirus E1A-conserved region 3 transactivation.Journal of virology · 2012Article
- Comparison of E1A CR3-dependent transcriptional activation across six different human adenovirus subgroups.Journal of virology · 2010Article
- Requirements for E1A dependent transcription in the yeast Saccharomyces cerevisiae.BMC molecular biology · 2009Article
- Transcriptional control by adenovirus E1A conserved region 3 via p300/CBP.Nucleic acids research · 2009Article
- Intrinsic structural disorder in adenovirus E1A: a viral molecular hub linking multiple diverse processes.Journal of virology · 2008Review
- Requirement of Sur2 for efficient replication of mouse adenovirus type 1.Journal of virology · 2004Article
- The adenovirus e3 promoter is sensitive to activation signals in human T cells.Journal of virology · 2003Article
- Clastosome: a subtype of nuclear body enriched in 19S and 20S proteasomes, ubiquitin, and protein substrates of proteasome.Molecular biology of the cell · 2002Article
- AR1 is an integral part of the adenovirus type 2 E1A-CR3 transactivation domain.Journal of virology · 1998Article
- A novel E1A domain mediates skeletal-muscle-specific enhancer repression independently of pRB and p300 binding.Molecular and cellular biology · 1996Article
- The CtBP binding domain in the adenovirus E1A protein controls CR1-dependent transactivation.Nucleic acids research · 1996Article
- Adenovirus E4 open reading frame 4 protein autoregulates E4 transcription by inhibiting E1A transactivation of the E4 promoter.Journal of virology · 1996Article
- E2F1 and E1A(12S) have a homologous activation domain regulated by RB and CBP.Proceedings of the National Academy of Sciences of the United States of America · 1996Article
- Targeting of adenovirus E1A and E4-ORF3 proteins to nuclear matrix-associated PML bodies.The Journal of cell biology · 1995Article
- The DNA binding domains of the yeast Gal4 and human c-Jun transcription factors interact through the zinc-finger and bZIP motifs.Nucleic acids research · 1995Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The adenovirus E1A protein contains three well conserved regions, designated conserved region (CR) 1, 2 and 3, which are important for the multiple activities ascribed to E1A. The CR3 domain constitutes a prototypic transcription activator, consisting of a promoter targeting region and a transactivating region. Here we demonstrate the existence of a second transactivating region located within amino acids 28 to 90 (essentially the CR1 domain) of the E1A protein. A fusion protein, containing the Gal4 DNA binding domain linked to CR1, was as efficient as the classical CR3 transactivator in activating transcription from a reporter plasmid containing Gal4 binding sites. However, competition experiments suggest that Gal/CR1 and Gal/CR3 work through different cellular targets. The E1A-243R protein has previously been extensively characterized as a repressor of transcription. Here we show that a Gal4 fusion protein expressing the CR1 domain is indeed sufficient for repression of SV40 enhancer activity. Collectively, our results suggest that CR1 functions as an activator if tethered to a promoter and as a repressor in the absence of promoter association.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.