ArticleNucleic acids research1994
Repression of transcriptional activity at a distance by the evolutionarily conserved KRAB domain present in a subfamily of zinc finger proteins.
Article in Nucleic acids research, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
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40 citing papers in PubMed, 132 citations in OpenAlex.
- SUMOylation of Bonus, theeLife · 2023Article
- A Comprehensive Review on the Role of ZSCAN4 in Embryonic Development, Stem Cells, and Cancer.Stem cell reviews and reports · 2022Review
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- Structures and biological functions of zinc finger proteins and their roles in hepatocellular carcinoma.Biomarker research · 2022Review
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- Apobec3A maintains HIV-1 latency through recruitment of epigenetic silencing machinery to the long terminal repeat.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Role of the zinc finger and SCAN domain-containing transcription factors in cancer.American journal of cancer research · 2019Review
- Molecular structures guide the engineering of chromatin.Nucleic acids research · 2017Article
- Deep vertebrate roots for mammalian zinc finger transcription factor subfamilies.Genome biology and evolution · 2014Article
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- Splice variants of zinc finger protein 695 mRNA associated to ovarian cancer.Journal of ovarian research · 2013Article
- Article
- Structural analysis and dimerization profile of the SCAN domain of the pluripotency factor Zfp206.Nucleic acids research · 2012Article
- Crystal optimization and preliminary diffraction data analysis of the SCAN domain of Zfp206.Acta crystallographica. Section F, Structural biology and crystallization communications · 2012Article
- Gypsy and the birth of the SCAN domain.Journal of virology · 2011Article
- Article
- Article
- Epigenetic gene silencing by the SRY protein is mediated by a KRAB-O protein that recruits the KAP1 co-repressor machinery.The Journal of biological chemistry · 2009Article
- A transcript profiling approach reveals the zinc finger transcription factor ZNF191 is a pleiotropic factor.BMC genomics · 2009Article
- Structure of the SCAN domain from the tumor suppressor protein MZF1.Journal of molecular biology · 2006Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Sub-families of related zinc finger protein genes have been defined on the basis of evolutionarily conserved structural features found outside the C2-H2 finger repeats. Such elements include the FAX domain found in a large number of Xenopus ZFPs, the evolutionarily conserved KRAB (Krüppel-associated box) and the ZiN (zinc finger N-terminal) domains. Here we describe a new evolutionarily conserved motif within zinc finger proteins which we have named the leucine rich region (LeR). Since conserved modules in regulatory proteins may specify properties relevant to their action we have determined the functional capabilities of LeR and the KRAB domains in the regulation of gene transcription by fusing relevant regions to a heterologous DNA-binding domain (GAL4 DNA-binding domain). We found that the KRAB-A domain tethered to RNA polymerase II promoters by a GAL4 DNA-binding domain actively represses transcription in a distance-independent manner. KRAB-mediated repression is dependent on the dose of the GAL4-KRAB-A fusion protein and on the presence of GAL4 binding sites on the DNA. Conversely, the LeR domain did not modulate significantly the transcription. Our results indicate that the KRAB domain present in the non-finger region of many ZFP genes quenches transcription possibly due to specific protein-protein interactions between the KRAB-A domain and components of the proximal transcriptional apparatus.
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