ArticleMolecular and cellular biology1994
A novel DNA replication origin identified in the human heat shock protein 70 gene promoter.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed, 52 citations in OpenAlex.
- A predictable conserved DNA base composition signature defines human core DNA replication origins.Nature communications · 2020Article
- Why are we where we are? Understanding replication origins and initiation sites in eukaryotes using ChIP-approaches.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- Transient dsDNA breaks during pre-replication complex assembly.Nucleic acids research · 2009Article
- The Mre11 complex mediates the S-phase checkpoint through an interaction with replication protein A.Molecular and cellular biology · 2007Article
- Replication-associated strand asymmetries in mammalian genomes: toward detection of replication origins.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- SV40 T antigen interacts with Nbs1 to disrupt DNA replication control.Genes & development · 2004Article
- Heterochromatin on the inactive X chromosome delays replication timing without affecting origin usage.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Deregulated c-Myc prematurely recruits both Type I and II CD95/Fas apoptotic pathways associated with terminal myeloid differentiation.Oncogene · 2002Article
- Long-distance control of origin choice and replication timing in the human beta-globin locus are independent of the locus control region.Molecular and cellular biology · 2000Article
- Interference of the simian virus 40 origin of replication by the cytomegalovirus immediate early gene enhancer: evidence for competition of active regulatory chromatin conformation in a single domain.Molecular and cellular biology · 2000Article
- Initiation of DNA replication at CpG islands in mammalian chromosomes.The EMBO journal · 1998Article
- A novel G1-specific enhancer identified in the human heat shock protein 70 gene.Nucleic acids research · 1997Article
- Induction of DNA replication by transcription in the region upstream of the human c-myc gene in a model replication system.Molecular and cellular biology · 1996Article
- Identification of an origin of bidirectional DNA replication in the ubiquitously expressed mammalian CAD gene.Molecular and cellular biology · 1995Article
- Site-specific initiation of DNA replication in Xenopus egg extract requires nuclear structure.Molecular and cellular biology · 1995Article
- Mapping of replication initiation sites in human ribosomal DNA by nascent-strand abundance analysis.Molecular and cellular biology · 1995Article
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3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A general and sensitive method for the mapping of initiation sites of DNA replication in vivo, developed by Vassilev and Johnson, has revealed replication origins in the region of simian virus 40 ori, in the regions upstream from the human c-myc gene and downstream from the Chinese hamster dihydrofolate reductase gene, and in the enhancer region of the mouse immunoglobulin heavy-chain gene. Here we report that the region containing the promoter of the human heat shock protein 70 (hsp70) gene was identified as a DNA replication origin in HeLa cells by this method. Several segments of the region were cloned into pUC19 and examined for autonomously replicating sequence (ARS) activity. The plasmids carrying the segments replicated episomally and semiconservatively when transfected into HeLa cells. The segments of ARS activity contained the sequences previously identified as binding sequences for a c-myc protein complex (T. Taira, Y. Negishi, F. Kihara, S. M. M. Iguchi-Ariga, and H. Ariga, Biochem. Biophys. Acta 1130:166-174, 1992). Mutations introduced within the c-myc protein complex binding sequences abolished the ARS activity. Moreover, the ARS plasmids stably replicated at episomal state for a long time in established cell lines. The results suggest that the promoter region of the human hsp70 gene plays a role in DNA replication as well as in transcription.
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