ArticleMolecular and cellular biology1994
Interactions between highly conserved U2 small nuclear RNA structures and Prp5p, Prp9p, Prp11p, and Prp21p proteins are required to ensure integrity of the U2 small nuclear ribonucleoprotein in Saccharomyces cerevisiae.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 55 citations in OpenAlex.
- Structural and functional modularity of the U2 snRNP in pre-mRNA splicing.Critical reviews in biochemistry and molecular biology · 2019Review
- Functional roles of DExD/H-box RNA helicases in Pre-mRNA splicing.Journal of biomedical science · 2015Review
- A novel mechanism for Prp5 function in prespliceosome formation and proofreading the branch site sequence.Genes & development · 2015Article
- Malleable ribonucleoprotein machine: protein intrinsic disorder in the Saccharomyces cerevisiae spliceosome.PeerJ · 2013Article
- Structure and assembly of the SF3a splicing factor complex of U2 snRNP.The EMBO journal · 2012Article
- DExD/H-box Prp5 protein is in the spliceosome during most of the splicing cycle.RNA (New York, N.Y.) · 2009Article
- Identification of genes that function in the biogenesis and localization of small nucleolar RNAs in Saccharomyces cerevisiae.Molecular and cellular biology · 2008Article
- U2 toggles iteratively between the stem IIa and stem IIc conformations to promote pre-mRNA splicing.Genes & development · 2007Article
- Rearrangement of competing U2 RNA helices within the spliceosome promotes multiple steps in splicing.Genes & development · 2007Article
- A previously unidentified activity of yeast and mouse RNA:pseudouridine synthases 1 (Pus1p) on tRNAs.RNA (New York, N.Y.) · 2006Article
- U2 snRNA-protein contacts in purified human 17S U2 snRNPs and in spliceosomal A and B complexes.Molecular and cellular biology · 2006Article
- Prp5 bridges U1 and U2 snRNPs and enables stable U2 snRNP association with intron RNA.The EMBO journal · 2004Article
- ATP requirement for Prp5p function is determined by Cus2p and the structure of U2 small nuclear RNA.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Comparative analysis of editosome proteins in trypanosomatids.Nucleic acids research · 2003Article
- Characterization of novel SF3b and 17S U2 snRNP proteins, including a human Prp5p homologue and an SF3b DEAD-box protein.The EMBO journal · 2002Article
- Domains in human splicing factors SF3a60 and SF3a66 required for binding to SF3a120, assembly of the 17S U2 snRNP, and prespliceosome formation.Molecular and cellular biology · 2001Article
- The ATP requirement for U2 snRNP addition is linked to the pre-mRNA region 5' to the branch site.RNA (New York, N.Y.) · 2001Article
- Sequences upstream of the branch site are required to form helix II between U2 and U6 snRNA in a trans-splicing reaction.Nucleic acids research · 2001Article
- Identification and characterization of yUAP/Sub2p, a yeast homolog of the essential human pre-mRNA splicing factor hUAP56.Genes & development · 2001Article
- Functional Cus1p is found with Hsh155p in a multiprotein splicing factor associated with U2 snRNA.Molecular and cellular biology · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Binding of U2 small nuclear ribonucleoprotein (snRNP) to the pre-mRNA is an early and important step in spliceosome assembly. We searched for evidence of cooperative function between yeast U2 small nuclear RNA (snRNA) and several genetically identified splicing (Prp) proteins required for the first chemical step of splicing, using the phenotype of synthetic lethality. We constructed yeast strains with pairwise combinations of 28 different U2 alleles with 10 prp mutations and found lethal double-mutant combinations with prp5, -9, -11, and -21 but not with prp3, -4, -8, or -19. Many U2 mutations in highly conserved or invariant RNA structures show no phenotype in a wild-type PRP background but render mutant prp strains inviable, suggesting that the conserved but dispensable U2 elements are essential for efficient cooperative function with specific Prp proteins. Mutant U2 snRNA fails to accumulate in synthetic lethal strains, demonstrating that interaction between U2 RNA and these four Prp proteins contributes to U2 snRNP assembly or stability. Three of the proteins (Prp9p, Prp11p, and Prp21p) are associated with each other and pre-mRNA in U2-dependent splicing complexes in vitro and bind specifically to synthetic U2 snRNA added to crude splicing extracts depleted of endogenous U2 snRNPs. Taken together, the results suggest that Prp9p, -11p, and -21p are U2 snRNP proteins that interact with a structured region including U2 stem loop IIa and mediate the association of the U2 snRNP with pre-mRNA.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.