ArticleMolecular and cellular biology1994
Position independence and proper developmental control of gamma-globin gene expression require both a 5' locus control region and a downstream sequence element.
Article in Molecular and cellular biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
34 citing papers in PubMed, 192 citations in OpenAlex.
- Trial
- Article
- Original Research: Generation of non-deletional hereditary persistence of fetal hemoglobin β-globin locus yeast artificial chromosome transgenic mouse models: -175 Black HPFH and -195 Brazilian HPFH.Experimental biology and medicine (Maywood, N.J.) · 2016Article
- The human ankyrin 1 promoter insulator sustains gene expression in a β-globin lentiviral vector in hematopoietic stem cells.Molecular therapy. Methods & clinical development · 2015Article
- LCR 5' hypersensitive site specificity for globin gene activation within the active chromatin hub.Nucleic acids research · 2012Article
- Inter-MAR association contributes to transcriptionally active looping events in human beta-globin gene cluster.PloS one · 2009Article
- Article
- Autonomous silencing as well as competition controls gamma-globin gene expression during development.Molecular and cellular biology · 2006Article
- Genome architecture of the human beta-globin locus affects developmental regulation of gene expression.Molecular and cellular biology · 2005Article
- Differences of globin transgene expression in stably transfected cell lines and transgenic mice.Blood · 2005Article
- The minimal promoter plays a major role in silencing of the galago gamma-globin gene in adult erythropoiesis.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Developmentally specific role of the CCAAT box in regulation of human gamma-globin gene expression.The Journal of biological chemistry · 2004Article
- Article
- Mutation of a transcriptional motif of a distant regulatory element reduces the expression of embryonic and fetal globin genes.Human molecular genetics · 2003Article
- Sequences in the (A)gamma-delta intergenic region are not required for stage-specific regulation of the human beta-globin gene locus.Proceedings of the National Academy of Sciences of the United States of America · 2003Article
- Locus control regions.Blood · 2002Review
- Evidence that DNase I hypersensitive site 5 of the human beta-globin locus control region functions as a chromosomal insulator in transgenic mice.Nucleic acids research · 2002Article
- Developmental specificity of recruitment of TBP to the TATA box of the human gamma-globin gene.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- A high-capacity, capsid-modified hybrid adenovirus/adeno-associated virus vector for stable transduction of human hematopoietic cells.Journal of virology · 2002Article
- Regulation of embryonic/fetal globin genes by nuclear hormone receptors: a novel perspective on hemoglobin switching.The EMBO journal · 1999Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
We have analyzed the expression of human gamma-globin genes during development in F2 progeny of transgenic mice carrying two types of constructs. In the first type, gamma-globin genes were linked individually to large (approximately 4-kb) sequence fragments spanning locus control region (LCR) hypersensitive site 2 (HS2) or HS3. These LCR fragments contained not only the core HS elements but also extensive evolutionarily conserved flanking sequences. The second type of construct contained tandem gamma- and beta-globin genes linked to identical HS2 or HS3 fragments. We show that gamma-globin expression in transgenic mice carrying HS2 gamma or HS3 gamma constructs is highly sensitive to position effects and that such effects override the cis regulatory elements present in these constructs to produce markedly different developmental patterns of gamma-globin expression in lines carrying the same transgene. In contrast, gamma-globin expression in both HS2 gamma beta and HS3 gamma beta mice is sheltered from position effects and the developmental patterns of gamma-globin expression in lines carrying the same transgene are identical and display stage-specific regulation. The results suggest that cis regulatory sequences required for proper developmental control of fetal globin expression in the presence of an LCR element reside downstream from the gamma genes.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.