ArticleJournal of clinical pathology1994
Sources of DNA for detecting B cell monoclonality using PCR.
Article in Journal of clinical pathology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed, 1 synthesis or guideline pooled it, 128 citations in OpenAlex.
- Molecular analysis of H&E- and Papanicolau-stained samples-systematic review.Histochemistry and cell biology · 2020Pooled it
- Fixed nuclei as alternative template of BIOMED-2 multiplex polymerase chain reaction for immunoglobulin gene clonality testing in B-cell malignancies.American journal of blood research · 2015Article
- A simple and cost-effective method of DNA extraction from small formalin-fixed paraffin-embedded tissue for molecular oncologic testing.BMC clinical pathology · 2014Article
- No evidence for interference of h&e staining in DNA testing: usefulness of DNA extraction from H&E-stained archival tissue sections.American journal of clinical pathology · 2012Article
- Comparison of germinal center markers CD10, BCL6 and human germinal center-associated lymphoma (HGAL) in follicular lymphomas.Diagnostic pathology · 2011Article
- Allele-specific transcriptional elongation regulates monoallelic expression of the IGF2BP1 gene.Epigenetics & chromatin · 2011Article
- Polymerase chain reaction (PCR) detection of B cell clonality in Sjögren's syndrome patients: a diagnostic tool of clonal expansion.Clinical and experimental immunology · 2010Article
- Polymerase chain reaction-based clonality analysis of cutaneous B-cell lymphoproliferative processes.Clinics (Sao Paulo, Brazil) · 2010Article
- Coexistence of splenic marginal zone lymphoma with hepatocellular carcinoma: a case report.Diagnostic pathology · 2007Article
- Protocol for the use of polymerase chain reaction in the detection of intraocular large B-cell lymphoma in ocular samples.The Journal of molecular diagnostics : JMD · 2007Article
- Optimization of PCR amplification for B- and T-cell clonality analysis on formalin-fixed and paraffin-embedded samples.Pathology oncology research : POR · 2007Article
- How we process trephine biopsy specimens: epoxy resin embedded bone marrow biopsies.Journal of clinical pathology · 2005Article
- B-cell clonality determination using an immunoglobulin kappa light chain polymerase chain reaction method.The Journal of molecular diagnostics : JMD · 2005Article
- Improvements to B cell clonality analysis using PCR amplification of immunoglobulin light chain genes.Molecular pathology : MP · 2002Article
- Demonstration of light chain restricted clonal B-lymphoid infiltrates in archival bone marrow trephines by quantitative real-time polymerase chain reaction.The American journal of pathology · 2001Article
- Epstein-Barr virus infection in paediatric liver transplant recipients: detection of the virus in post-transplant tonsillectomy specimens.Molecular pathology : MP · 2001Article
- Polymerase chain reaction detection of Kaposi's sarcoma-associated herpesvirus-optimized protocols and their application to myeloma.The Journal of molecular diagnostics : JMD · 2001Article
- Consistent patterns of allelic loss in natural killer cell lymphoma.The American journal of pathology · 2000Article
- Parvovirus B19 is associated with benign testes as well as testicular germ cell tumours.Molecular pathology : MP · 1999Article
- Detection of clonal B cells in microdissected reactive lymphoproliferations: possible diagnostic pitfalls in PCR analysis of immunoglobulin heavy chain gene rearrangement.Molecular pathology : MP · 1999Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
aimsTo evaluate the polymerase chain reaction (PCR) demonstration of clonal immunoglobulin heavy chain gene rearrangements using routinely prepared, unstained, and stained formalin fixed, paraffin wax embedded tissue samples.
methodsExtracts from (a) fresh frozen tissue samples, (b) unstained, and (c) haematoxylin and eosin stained formalin fixed, paraffin wax embedded 5 microns tissue sections from 42 cases of low grade B cell lymphoma, all shown to be monoclonal by Southern blot analysis, were analysed using PCR. Two regions of the variable segment of the immunoglobulin heavy chain gene were amplified (framework 2 to joining region [Fr2/JH] and framework 3 to joining region [Fr3/JH]). Twelve samples of reactive lymphoid tissue were studied as controls. Products from each case were directly compared on polyacrylamide gels.
resultsUsing both primer combinations, monoclonality was detected in 38 of 42 (90%) cases using fresh material, 37 of 42 (88%) using unstained paraffin wax embedded samples, and in 35 of 42 (83%) cases using haematoxylin and eosin stained sections. No false positive results attributable to fixation, processing, or staining were identified, although the efficiency of amplification using the Fr2/JH primers was significantly reduced.
conclusionsPCR determination of B cell clonality using paraffin wax embedded material is sufficiently sensitive and reliable for use as a routine diagnostic adjunct to conventional morphological and immunocytochemical assessment of lymphoproliferative disease.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.