ArticleThe Journal of cell biology1994
Characterization of an F-actin-binding domain in the cytoskeletal protein vinculin.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
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Who cites it
45 citing papers in PubMed, 154 citations in OpenAlex.
- Reconstituted nascent adhesion condensates drive actin polymerization on supported lipid bilayers.Science advances · 2026Article
- The actin binding sites of talin have both distinct and complementary roles in cell-ECM adhesion.PLoS genetics · 2024Article
- Vinculin Y822 is an important determinant of ligand binding.Journal of cell science · 2023Article
- Understanding the molecular basis of cardiomyopathy.American journal of physiology. Heart and circulatory physiology · 2022Review
- Vinculin-mediated axon growth requires interaction with actin but not talin in mouse neocortical neurons.Cellular and molecular life sciences : CMLS · 2021Article
- Mechanical stability of αT-catenin and its activation by force for vinculin binding.Molecular biology of the cell · 2019Article
- Distinct focal adhesion protein modules control different aspects of mechanotransduction.Journal of cell science · 2017Article
- Action and Traction: Cytoskeletal Control of Receptor Triggering at the Immunological Synapse.Frontiers in immunology · 2016Review
- Phosphorylation at Y1065 in vinculin mediates actin bundling, cell spreading, and mechanical responses to force.Biochemistry · 2014Article
- Identification of an actin binding surface on vinculin that mediates mechanical cell and focal adhesion properties.Structure (London, England : 1993) · 2014Article
- Vinculin and Rab5 complex is required [correction of requited]for uptake of Staphylococcus aureus and interleukin-6 expression.PloS one · 2014Article
- Rho-associated protein kinase modulates neurite extension by regulating microtubule remodeling and vinculin distribution.Neural regeneration research · 2013Article
- How vinculin regulates force transmission.Proceedings of the National Academy of Sciences of the United States of America · 2013Article
- Finding the weakest link: exploring integrin-mediated mechanical molecular pathways.Journal of cell science · 2012Review
- Myosin II activity dependent and independent vinculin recruitment to the sites of E-cadherin-mediated cell-cell adhesion.BMC cell biology · 2011Article
- New insights into vinculin function and regulation.International review of cell and molecular biology · 2011Review
- Vinculin is a dually regulated actin filament barbed end-capping and side-binding protein.The Journal of biological chemistry · 2010Article
- Dynamics of leading lamellae of living fibroblasts visualized by high-speed scanning probe microscopy.Histochemistry and cell biology · 2010Article
- Vinculin nucleates actin polymerization and modifies actin filament structure.The Journal of biological chemistry · 2009Article
- Molecular dynamics study of talin-vinculin binding.Biophysical journal · 2008Article
Corrections and comments
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Vinculin, a major structural component of vertebrate cell-cell and cell-matrix adherens junctions, has been found to interact with several other junctional components. In this report, we have identified and characterized a binding site for filamentous actin. These results included studies with gizzard vinculin, its proteolytic head and tail fragments, and recombinant proteins containing various gizzard vinculin sequences fused to the maltose binding protein (MBP) of Escherichia coli. In cosedimentation assays, only the vinculin tail sequence mediated a direct interaction with actin filaments. The binding was saturable, with a dissociation constant value in the micromolar range. Experiments with deletion clones localized the actin-binding domain to a region confined by residues 893-1016 in the 170-residue-long carboxyterminal segment, while the proline-rich hinge connecting the globular head to the rodlike tail was not required for this interaction. In fixed and permeabilized cells (cell models), as well as after microinjection, proteins containing the actin-binding domain specifically decorated stress fibers and the cortical network of fibroblasts and epithelial cells, as well as of brush border type microvilli. These results corroborated the sedimentation experiments. Our data support and extend previous work showing that vinculin binds directly to actin filaments. They are consistent with a model suggesting that in adhesive cells, the NH2-terminal head piece of vinculin directs this molecule to the focal contact sites, while its tail segment causes bundling of the actin filament ends into the characteristic spear tip-shaped structures.
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