ArticleThe Journal of cell biology1994
pH-independent and -dependent cleavage of proinsulin in the same secretory vesicle.
Article in The Journal of cell biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 74 citations in OpenAlex.
- Decoding Insulin Secretory Granule Maturation Using Genetically Encoded pH Sensors.ACS sensors · 2024Article
- Understanding Insulin in the Age of Precision Medicine and Big Data: Under-Explored Nature of Genomics.Biomolecules · 2023Review
- The Pancreatic β-Cell: The Perfect Redox System.Antioxidants (Basel, Switzerland) · 2021Review
- Regulation of Glucose-Dependent Golgi-Derived Microtubules by cAMP/EPAC2 Promotes Secretory Vesicle Biogenesis in Pancreatic β Cells.Current biology : CB · 2019Article
- Activation of GLP-1 receptor signalling alleviates cellular stresses and improves beta cell function in a mouse model of Wolfram syndrome.Diabetologia · 2018Article
- Loss of mTORC1 signalling impairs β-cell homeostasis and insulin processing.Nature communications · 2017Article
- HID-1 is required for homotypic fusion of immature secretory granules during maturation.eLife · 2016Article
- A Novel GLP1 Receptor Interacting Protein ATP6ap2 Regulates Insulin Secretion in Pancreatic Beta Cells.The Journal of biological chemistry · 2015Article
- The Ras/cAMP/protein kinase A pathway regulates glucose-dependent assembly of the vacuolar (H+)-ATPase in yeast.The Journal of biological chemistry · 2008Article
- Secretory vesicle rebound hyperacidification and increased quantal size resulting from prolonged methamphetamine exposure.Journal of neurochemistry · 2008Article
- Amylin proprotein processing generates progressively more amyloidogenic peptides that initially sample the helical state.Biochemistry · 2008Article
- Mutant proinsulin that cannot be converted is secreted efficiently from primary rat beta-cells via the regulated pathway.Molecular biology of the cell · 2003Article
- Stimulation-dependent regulation of the pH, volume and quantal size of bovine and rodent secretory vesicles.The Journal of physiology · 2002Article
- The luminal part of the murine cytomegalovirus glycoprotein gp40 catalyzes the retention of MHC class I molecules.The EMBO journal · 2000Article
- Insulinoma with hyperproinsulinemia during hypoglycemia and loss of expression of vacuolar-type H(+)-ATPase (V-ATPase) in the tumor tissue.International journal of pancreatology : official journal of the International Association of Pancreatology · 1999Article
- Homotypic fusion of immature secretory granules during maturation in a cell-free assay.The Journal of cell biology · 1998Article
- Biogenesis of secretory granules in the trans-Golgi network of neuroendocrine and endocrine cells.Biochimica et biophysica acta · 1998Review
- Distinct molecular events during secretory granule biogenesis revealed by sensitivities to brefeldin A.Molecular biology of the cell · 1997Article
- Differential sorting of lysosomal enzymes out of the regulated secretory pathway in pancreatic beta-cells.The Journal of cell biology · 1997Article
- pH-dependent processing of secretogranin II by the endopeptidase PC2 in isolated immature secretory granules.The Biochemical journal · 1997Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 1 institution in 2 countries.
Funding
Abstract
By quantitative immunoelectron microscopy and HPLC, we have studied the effect of disrupting pH gradients, by ammonium chloride, on proinsulin conversion in the insulin-producing B-cells of the islets of langerhans. Proinsulin content and pH in single secretory vesicles were measured on consecutive serial sections immunostained alternately with anti-proinsulin or anti-dinitrophenol (to reveal the pH-sensitive probe DAMP) antibodies. Radioactivity labeled proinsulin, proinsulin cleavage intermediates, and insulin were quantitated by HPLC analysis of extracts of islets treated in the same conditions. Cleavage at the C-peptide/A-chain junction is significantly less sensitive to pH gradient disruption than that of the B-chain/C-peptide junction, but the range of pH and proinsulin content in individual vesicles indicate that both cleavages occur in the same vesicle released from the TGN.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.