ArticleThe EMBO journal1994
A Chlamydomonas protein that binds single-stranded G-strand telomere DNA.
Article in The EMBO journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 48 citations in OpenAlex.
- A novel G·G·T non-conventional intramolecular triplex formed by the double repeat sequence ofRSC advances · 2022Article
- Molecular characterization ofLife science alliance · 2019Article
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- Screening a cDNA library for protein-protein interactions directly in planta.The Plant cell · 2012Article
- The role of an E-box element: multiple frunctions and interacting partners.Plant signaling & behavior · 2010Review
- Multiple roles and interaction factors of an E-box element in Chlamydomonas reinhardtii.Plant physiology · 2010Article
- POT1-independent single-strand telomeric DNA binding activities in Brassicaceae.The Plant journal : for cell and molecular biology · 2009Article
- NAB1 is an RNA binding protein involved in the light-regulated differential expression of the light-harvesting antenna of Chlamydomonas reinhardtii.The Plant cell · 2005Article
- Novel DNA-binding properties of the RNA-binding protein TIAR.Nucleic acids research · 2005Article
- Molecular map of the Chlamydomonas reinhardtii nuclear genome.Eukaryotic cell · 2003Article
- Telomere-binding and Stn1p-interacting activities are required for the essential function of Saccharomyces cerevisiae Cdc13p.Nucleic acids research · 2000Article
- Characterization and developmental expression of single-stranded telomeric DNA-binding proteins from mung bean (Vigna radiata).Plant molecular biology · 2000Article
- The Est1 subunit of yeast telomerase binds the Tlc1 telomerase RNA.Molecular and cellular biology · 2000Article
- Gbp1p, a protein with RNA recognition motifs, binds single-stranded telomeric DNA and changes its binding specificity upon dimerization.Molecular and cellular biology · 1999Article
- Rice proteins that bind single-stranded G-rich telomere DNA.Plant molecular biology · 1998Article
- Functionally interacting telomerase RNAs in the yeast telomerase complex.Genes & development · 1997Article
- Evidence for a HeLa nuclear protein that binds specifically to the single-stranded d(CCCTAA)n telomeric motif.Nucleic acids research · 1996Article
- The telobox, a Myb-related telomeric DNA binding motif found in proteins from yeast, plants and human.Nucleic acids research · 1996Article
- A class of single-stranded telomeric DNA-binding proteins required for Rap1p localization in yeast nuclei.Proceedings of the National Academy of Sciences of the United States of America · 1995Article
- Isolation and characterization of two Saccharomyces cerevisiae genes that encode proteins that bind to (TG1-3)n single strand telomeric DNA in vitro.Nucleic acids research · 1994Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have identified a protein in Chlamydomonas reinhardtii cell extracts that specifically binds the single-stranded (ss) Chlamydomonas G-strand telomere sequence (TTTTAGGG)n. This protein, called G-strand binding protein (GBP), binds DNA with two or more ss TTTTAGGG repeats. A single polypeptide (M(r) 34 kDa) in Chlamydomonas extracts binds (TTTTAGGG)n, and a cDNA encoding this G-strand binding protein was identified by its expression of a G-strand binding activity. The cDNA (GBP1) sequence predicts a protein product (Gbp1p) that includes two domains with extensive homology to RNA recognition motifs (RRMs) and a region rich in glycine, alanine and arginine. Antibody raised against a peptide within Gbp1p reacted with both the 34 kDa polypeptide and bound G-strand DNA-protein complexes in gel retardation assays, indicating that GBP1 encodes GBP. Unlike vertebrate heteronuclear ribonucleoproteins, GBP does not bind the cognate telomere RNA sequence UUUUAGGG in gel retardation, North-Western or competition assays. Thus, GBP is a new type of candidate telomere binding protein that binds, in vitro, to ss G-strand telomere DNA, the primer for telomerase, and has domains that have homology to RNA binding domains in other proteins.
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