ArticleThe Plant cell1994
A calmodulin-sensitive interaction between microtubules and a higher plant homolog of elongation factor-1 alpha.
Article in The Plant cell, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 52 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
52 citing papers in PubMed, 179 citations in OpenAlex.
- Proteome and Nutritional Shifts Observed in Hordein Double-Mutant Barley Lines.Frontiers in plant science · 2021Article
- Comparative Proteomics Analyses of Pollination Response in Endangered Orchid Species Dendrobium Chrysanthum.International journal of molecular sciences · 2017Article
- The Cytoskeleton and Its Regulation by Calcium and Protons.Plant physiology · 2016Review
- Holophytochrome-Interacting Proteins in Physcomitrella: Putative Actors in Phytochrome Cytoplasmic Signaling.Frontiers in plant science · 2016Article
- Multifunctional Microtubule-Associated Proteins in Plants.Frontiers in plant science · 2016Review
- Parvulin 17-catalyzed Tubulin Polymerization Is Regulated by Calmodulin in a Calcium-dependent Manner.The Journal of biological chemistry · 2015Article
- Article
- Isolation and characterization of three cassava elongation factor 1 alpha (MeEF1A) promoters.PloS one · 2014Article
- Thermal-stable proteins of fruit of long-living Sacred LotusTropical plant biology · 2013Article
- Characterization and functional analysis of the potato pollen-specific microtubule-associated protein SBgLR in tobacco.PloS one · 2013Article
- The cell biology of Tobacco mosaic virus replication and movement.Frontiers in plant science · 2013Article
- Proteomic analysis of the low mutation rate of diploid male gametes induced by colchicine in Ginkgo biloba L.PloS one · 2013Article
- The role of retrograde intraflagellar transport in flagellar assembly, maintenance, and function.The Journal of cell biology · 2012Article
- Lysine acetylation is a widespread protein modification for diverse proteins in Arabidopsis.Plant physiology · 2011Article
- eEF1A: thinking outside the ribosome.The Journal of biological chemistry · 2010Review
- The RACK1 signal anchor protein from Trypanosoma brucei associates with eukaryotic elongation factor 1A: a role for translational control in cytokinesis.Molecular microbiology · 2008Article
- The nuclear migration protein NUDF/LIS1 forms a complex with NUDC and BNFA at spindle pole bodies.Eukaryotic cell · 2008Article
- Molecular characterization and expression analysis of five different elongation factor 1 alpha genes in the flatfish Senegalese sole (Solea senegalensis Kaup): differential gene expression and thyroid hormones dependence during metamorphosis.BMC molecular biology · 2008Article
- Elongation factor 1a mediates the specificity of mitochondrial tRNA import in T. brucei.The EMBO journal · 2007Article
- Microtubule-associated proteins in higher plants.Journal of plant research · 2007Review
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The microtubules (MTs) of higher plant cells are organized into arrays with essential functions in plant cell growth and differentiation; however, molecular mechanisms underlying the organization and regulation of these arrays remain largely unknown. We have approached this problem using tubulin affinity chromatography to isolate carrot proteins that interact with MTs. From these proteins, a 50-kD polypeptide was selectively purified by exploiting its Ca(2+)-dependent binding to calmodulin (CaM). This polypeptide was identified as a homolog of elongation factor-1 alpha (EF-1 alpha)--a highly conserved and ubiquitous protein translation factor. The carrot EF-1 alpha homolog bundles MTs in vitro, and moreover, this bundling is modulated by the addition of Ca2+ and CaM together (Ca2+/CaM). A direct binding between the EF-1 alpha homolog and MTs was demonstrated, providing novel evidence for such an interaction. Based on these findings, and others discussed herein, we propose that an EF-1 alpha homolog mediates the lateral association of MTs in plant cells by a Ca2+/CaM-sensitive mechanism.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.