ArticleBiophysical journal1994
Quantitative study of molecular transport due to electroporation: uptake of bovine serum albumin by erythrocyte ghosts.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 60 citations in OpenAlex.
- Intracellular Delivery by Membrane Disruption: Mechanisms, Strategies, and Concepts.Chemical reviews · 2018Review
- Calcein Release from Cells In Vitro via Reversible and Irreversible Electroporation.The Journal of membrane biology · 2018Article
- Mechanisms of transfer of bioactive molecules through the cell membrane by electroporation.European biophysics journal : EBJ · 2015Review
- Evidence for electro-induced membrane defects assessed by lateral mobility measurement of a GPi anchored protein.European biophysics journal : EBJ · 2014Article
- Selective cytotoxicity of intense nanosecond-duration electric pulses in mammalian cells.Biochimica et biophysica acta · 2010Article
- Kinetics of transmembrane transport of small molecules into electropermeabilized cells.Biophysical journal · 2008Article
- Mechanistic analysis of electroporation-induced cellular uptake of macromolecules.Experimental biology and medicine (Maywood, N.J.) · 2008Article
- The cell as a biomaterial.Journal of materials science. Materials in medicine · 2002Article
- Prediction and optimization of gene transfection and drug delivery by electroporation.Gene therapy · 2001Article
- Quantitative study of electroporation-mediated molecular uptake and cell viability.Biophysical journal · 2001Article
- Calcium waves induced by large voltage pulses in fish keratocytes.Biophysical journal · 1998Article
- Electropermeabilization of mammalian cells to macromolecules: control by pulse duration.Biophysical journal · 1998Article
- Energetic constraints on the creation of cell membrane pores by magnetic particles.Biophysical journal · 1996Article
- Millisecond measurement of transport during and after an electroporation pulse.Biophysical journal · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Electroporation is believed to involve the creation of aqueous pathways in lipid bilayer membranes by transient elevation of the transmembrane voltage to approximately 1 V. Here, results are presented for a quantitative study of the number of bovine serum albumin (BSA) molecules transported into erythrocyte ghosts caused by electroportion. 1) Uptake of BSA was found to plateau at high field strength. However, this was not necessarily an absolute maximum in transport. Instead, it represented the maximum effect of increasing field strength for a particular pulse protocol. 2) Maximum uptake under any conditions used in this study corresponded to approximately one-fourth of apparent equilibrium with the external solution. 3) Multiple and longer pulses each increased uptake of BSA, where the total time integral of field strength correlated with uptake, independent of inter-pulse spacing. 4) Pre-pulse adsorption of BSA to ghost membranes appears to have increased transport. 5) Most transport of BSA probably occurred by electrically driven transport during pulses; post-pulse uptake occurred, but to a much lesser extent. Finally, approaches to increasing transport are discussed.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.