ArticleBiophysical journal1994
Granula motion and membrane spreading during activation of human platelets imaged by atomic force microscopy.
Article in Biophysical journal, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Amyloid Protein-Induced Remodeling of Morphometry and Nanomechanics in Human Platelets.Biomedicines · 2025Article
- Evaluation of Surface Structure and Morphological Phenomena of Caucasian Virgin Hair with Atomic Force Microscopy.Medicina (Kaunas, Lithuania) · 2024Article
- Platelets' Nanomechanics and Morphology in Neurodegenerative Pathologies.Biomedicines · 2022Article
- Platelet Membrane: An Outstanding Factor in Cancer Metastasis.Membranes · 2022Review
- Quantifying single-platelet biomechanics: An outsider's guide to biophysical methods and recent advances.Research and practice in thrombosis and haemostasis · 2020Review
- Algal Viruses: The (Atomic) Shape of Things to Come.Viruses · 2018Review
- Rupture Forces among Human Blood Platelets at different Degrees of Activation.Scientific reports · 2016Article
- Activation induced morphological changes and integrin αIIbβ3 activity of living platelets.Methods (San Diego, Calif.) · 2013Article
- Granule exocytosis is required for platelet spreading: differential sorting of α-granules expressing VAMP-7.Blood · 2012Article
- Novel nanostructured biomaterials: implications for coronary stent thrombosis.International journal of nanomedicine · 2012Article
- Redox regulation of morphology, cell stiffness, and lectin-induced aggregation of human platelets.European biophysics journal : EBJ · 2011Article
- Noncontact microrheology at acoustic frequencies using frequency-modulated atomic force microscopy.Nature methods · 2010Article
- Nanomedicine for the reduction of the thrombogenicity of stent coatings.International journal of nanomedicine · 2010Article
- Spider silk softening by water uptake: an AFM study.European biophysics journal : EBJ · 2008Article
- Atomic force microscopy study of the specific adhesion between a colloid particle and a living melanoma cell: Effect of the charge and the hydrophobicity of the particle surface.Biophysical journal · 2006Article
- Cellular transfer and AFM imaging of cancer cells using Bioimprint.Journal of nanobiotechnology · 2006Article
- Physical morphology and surface properties of unsaturated Pseudomonas putida biofilms.Journal of bacteriology · 2000Article
- Drug-induced changes of cytoskeletal structure and mechanics in fibroblasts: an atomic force microscopy study.Biophysical journal · 2000Article
- Dimensional and mechanical dynamics of active and stable edges in motile fibroblasts investigated by using atomic force microscopy.Proceedings of the National Academy of Sciences of the United States of America · 1999Article
- Imaging of the surface of living cells by low-force contact-mode atomic force microscopy.Biophysical journal · 1998Article
Corrections and comments
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The redistribution of platelet constituents during activation is essential for their physiological function of maintaining hemostasis. We report here about real time investigations of the activation of native human platelets under physiological conditions from the initial formation of filopodia to the fully spread form by atomic force microscopy. We followed the trafficking of granules and their interaction with the plasma membrane within single cells. Our results show movement of certain granula towards the lamellipodia. Analysis of this rearrangement and the subsequent enlargement of the platelet surface reveals details of the membrane spreading process. Images of living cells are presented that show the distribution of cytoskeletal components and membrane-bound filaments at a resolution of better than 50 nm. The local minimum forces between the tip and the platelets were estimated to be smaller than 60 pN. A model for the elastic contributions of the glycocalix to the tip/membrane interaction was developed using the theory of grafted polymers.
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