ArticleThe Journal of membrane biology1994
G-protein activation mediates prepulse facilitation of Ca2+ channel currents in bovine chromaffin cells.
Article in The Journal of membrane biology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 51 citations in OpenAlex.
- Article
- Involvement of histaminergic inputs in the jaw-closing reflex arc.Journal of neurophysiology · 2015Article
- Importance of voltage-dependent inactivation in N-type calcium channel regulation by G-proteins.Pflugers Archiv : European journal of physiology · 2007Article
- Direct and remote modulation of L-channels in chromaffin cells: distinct actions on alpha1C and alpha1D subunits?Molecular neurobiology · 2004Review
- Opposite action of beta1- and beta2-adrenergic receptors on Ca(V)1 L-channel current in rat adrenal chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- Histamine promotes excitability in bovine adrenal chromaffin cells by inhibiting an M-current.The Journal of physiology · 2002Article
- Timing of dense-core vesicle exocytosis depends on the facilitation L-type Ca channel in adrenal chromaffin cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Facilitation of rabbit alpha1B calcium channels: involvement of endogenous Gbetagamma subunits.The Journal of physiology · 1998Article
- Structural features determining differential receptor regulation of neuronal Ca channels.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1998Article
- Voltage-dependent modulation of T-type calcium channels by protein tyrosine phosphorylation.The EMBO journal · 1997Article
- The mechanism of calcium channel facilitation in bovine chromaffin cells.The Journal of physiology · 1996Article
- Activation of delta-opioid receptors inhibits neuronal-like calcium channels and distal steps of Ca(2+)-dependent secretion in human small-cell lung carcinoma cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 1996Article
- Facilitation of T-type calcium current in bullfrog atrial cells: voltage-dependent relief of a G protein inhibitory tone.The Journal of physiology · 1996Article
- Modulation of calcium current by ATP in guinea-pig adrenal chromaffin cells.Pflugers Archiv : European journal of physiology · 1996Article
- ATP and G proteins affect the runup of the Ca2+ current in bovine chromaffin cells.Pflugers Archiv : European journal of physiology · 1995Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
The effects of G-protein activation were investigated on tonic, large depolarization-induced Ca2+ channel facilitation in cultured bovine adrenal chromaffin cells. Under whole-cell voltage clamp, activation of G proteins by intracellular dialysis with 200 microM GTP-gamma S did not significantly affect prepulse facilitation or whole-cell Ba2+ current (IBa) density. In contrast, inactivation of G proteins by intracellular GDP-beta S or pertussis toxin (PTX) pretreatment completely abolished or markedly attenuated facilitation of IBa, respectively. GDP-beta S dialysis resulted in nearly a threefold increase in peak IBa density, whereas PTX pretreatment resulted in a 50% increase. Our results indicate that under control recording conditions (200 microM intracellular GTP), G proteins are tonically activated and suppress high-voltage-activated (HVA) Ca2+ channels in a voltage-dependent and voltage-independent manner. Local superfusion of chromaffin cells with normal bath solution produced a rapid and reversible increase (approximately 50%) in IBa amplitudes that also abolished prepulse facilitation. Together, these results demonstrate that tonic facilitation of HVA Ca2+ channels in bovine chromaffin cells involves the voltage-dependent relief of a G-protein-mediated suppression, imposed by chromaffin cell secretory products that feedback and activate G-protein-coupled autoreceptors.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.