ArticleJournal of clinical microbiology1994
Partial characterization of Streptococcus suis type 2 hemolysin.
Article in Journal of clinical microbiology, 1994. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 23 citations in OpenAlex.
- Evaluation of Galleria mellonella larvae for studying the virulence of Streptococcus suis.BMC microbiology · 2016Article
- Different foreign genes incidentally integrated into the same locus of the Streptococcus suis genome.Journal of bacteriology · 2005Article
- Evidence for lateral transfer of the Suilysin gene region of Streptococcus suis.Journal of bacteriology · 2002Article
- Characterization of Streptococcus suis genes encoding proteins homologous to sortase of gram-positive bacteria.Journal of bacteriology · 2002Article
- Generation and characterization of a defined mutant of Streptococcus suis lacking suilysin.Infection and immunity · 2001Article
- Selection of recombinant antibodies specific for pathogenic Streptococcus suis by subtractive phage display.Infection and immunity · 2000Article
- Identification and characterization of the cps locus of Streptococcus suis serotype 2: the capsule protects against phagocytosis and is an important virulence factor.Infection and immunity · 1999Article
- Production of virulence-related proteins by Canadian strains of Streptococcus suis capsular type 2.Canadian journal of veterinary research = Revue canadienne de recherche veterinaire · 1998Article
- Use of ribotyping and hemolysin activity to identify highly virulent Streptococcus suis type 2 isolates.Journal of clinical microbiology · 1998Article
- Detection of genomic heterogeneity in Streptococcus suis isolates by DNA restriction fragment length polymorphisms of rRNA genes (ribotyping).Journal of clinical microbiology · 1995Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Streptococcus suis type 2 was evaluated for hemolysin production. Supernatants of S. suis type 2 grown in Todd-Hewitt broth were assayed for hemolytic activity by a photometric assay. Twenty-two additional serotypes of S. suis (1,3 to 22, and 1/2) were evaluated for hemolysin production; nine of them (1/2, 1, 4, 5, 14, 15, 17, 19, and 20) were positive. The effects of temperature, atmosphere, centrifugation, sonication, chemicals, bovine serum albumin, fetal calf serum, and enzymes on S. suis type 2 hemolysin activity were studied. Maximum hemolysis occurred after incubation in RPMI 1640 medium at 40 degrees C in 6% CO2 and after growth in Todd-Hewitt broth at 37 degrees C under anaerobic conditions. Hemolytic activity was absent after the addition of fetal calf serum and decreased after the addition of trypsin or amylase. However, treatment of erythrocytes with amylase or trypsin prior to incubation with supernatant also resulted in a decrease in hemolytic activity. The addition of bovine serum albumin caused increased hemolytic activity. Dipyridyl and EDTA had negligible effects on hemolysis. Hemolytic S. suis type 2 culture supernatant injected intraperitoneally failed to cause death in BALB/c mice. Data from our study indicate that S. suis type 2 hemolysin is a secreted or loosely cell bound, thermolabile molecule whose activity is growth condition dependent.
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